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https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2006, Vol. 33 ›› Issue (5): 1051-1054.

• 研究报告 • 上一篇    下一篇

苹果铁结合蛋白基因的克隆及表达特性

叶霞1, 2;陶建敏1;蔡斌华1;章镇1*   

  1. (1 南京农业大学园艺学院, 江苏南京210095; 2 河南黄河园林绿化工程有限公司, 河南郑州450003)
  • 收稿日期:2005-12-19 修回日期:2006-02-09 出版日期:2006-10-25 发布日期:2006-10-25

Cloning and Expression Characteristics of the Ferritin Gene from Malusdomestica Borkh.

Ye Xia1, 2;Tao Jianmin1;Cai Binhua1;Zhang Zhen1*   

  1. (1College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China; 2Henan Yellow River Gardening Limited Company, Zhengzhou, Henan 450003, China)
  • Received:2005-12-19 Revised:2006-02-09 Online:2006-10-25 Published:2006-10-25

摘要: 根据已报道的植物铁结合蛋白基因( ferritin) 的保守区域设计引物, 用RT2PCR 的方法从‘嘎拉’苹果叶片中获得铁结合蛋白基因的全长序列。该基因cDNA共有1 043个碱基, 其中编码区834bp, 编码277个氨基酸残基的蛋白质, 终止密码子后有209 bp左右的3’端尾随序列区。Southern杂交结果显示ferritin基因在‘嘎拉’苹果基因组中至少有7个拷贝存在。0.5 mmol·L - 1的柠檬酸铁处理‘嘎拉’苹果试管苗不同时间后的定量RT-PCR分析表明, ‘嘎拉’苹果的铁结合蛋白基因随着诱导时间的延长表达量增加, 说明该基因可能在转录水平上受铁诱导表达。

关键词: 苹果, 铁结合蛋白基因, 克隆, 表达

Abstract: A full-length cDNA sequence of apple ferritin ( iron-binding protein) gene was cloned from the leaves of Malus domestica Borkh. by RT-PCR method. The gene was named as Mdfer and its sequence was deposited in the GenBank (accession number DQ099429). The total length of Mdfer gene is 1 043 bp consisting of a 834 bp open reading frame (ORF) coding for 277 amino acids, and 209 bp non-coding 3’ region after the stop codon. Southern blotting analysis suggested that there were at least seven cop ies of ferritin gene in
the genome of Malus domestica Borkh. When the seedlings of Malus domestica were treated with 0.5 mmol·L - 1 iron-citrate from zero to six hours, a gradually increased expression level of the ferritin gene was observed.This result suggested that the exp ression ofM dfer gene was possibly regulated by iron at transcrip tional level.

Key words: Apple, Ferritin gene, Cloning, Expression

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