秋水仙素诱导文旦柚2n配子获得自交三倍体

园艺学报 ›› 2006, Vol. 33 ›› Issue (5): 1045-1047.

秋水仙素诱导文旦柚2n配子获得自交三倍体

杨晓伶¹;程舟¹;李珊¹;北岛宣²;长谷川耕二郎³

(¹同济大学生命科学与技术学院, 上海200092; ²日本京都大学附属农场, 大阪56920096, 日本; ³日本高知大学农学部, 高知78328502, 日本)

收稿日期:2005-11-06 修回日期:2006-02-23 出版日期:2006-10-25 发布日期:2006-10-25

Self-hybridized Triploids Derived from 2n Gametes Induced by Colchicine inPummelo

Yang Xiaoling¹;Cheng Zhou¹;Li Shan¹;Kitajima Akira²;Hasegawa Kojiro³

(¹School of Life Science and Technology, Tongji University, Shanghai 200092, China; ² Experimental Farm, Kyoto University,Osaka 56920096, Japan; ³Faculty of Agriculture, Kochi University, Kochi 78328502, Japan)

Received:2005-11-06 Revised:2006-02-23 Online:2006-10-25 Published:2006-10-25

摘要/Abstract

摘要： 以‘土佐文旦’柚成树为材料, 探讨了秋水仙素诱导2n配子的方法, 研究了秋水仙素浓度和处理时期对获得三倍体的影响。结果表明: 开花前1周用0.25%的秋水仙素处理花蕾, 可诱导2n配子自交亲和。经对发芽存活的自交后代植株的叶片细胞核DNA量检测和细胞学鉴定, 确定25%的自交后代植株为自交三倍体。通过秋水仙素诱导文旦2n配子是获得自交三倍体的有效途径。

关键词: 柚, 秋水仙素, 2n配子, 三倍体

Abstract: Tosa-Buntan pummelo 〔Citrus grandis (L. ) Osb. 〕was used to establish a method of 2n gametes induced by colchicine. The effects of colchicine concentration and its treatment period on the derived self-hybridized triploids were also analyzed. The results showed that 2n gametes with self-compatibility could be induced by treating the bud with 0.25% colchicine at 1 week before flowering. 25% of the alive F₁ plants were considered as the self-hybridized trip loids after the measurement of leave cellular nucleus DNA content and cytological identification. Therefore, it is the effective method to obtain the self-hybridized triploids derived from 2n gametes induced by colchicines in pummelo.

Key words: Pummelo, Colchicine, 2n gamete, Triploid

中图分类号:

S 666.3

杨晓伶;程舟;李珊;北岛宣;长谷川耕二郎. 秋水仙素诱导文旦柚2n配子获得自交三倍体[J]. 园艺学报, 2006, 33(5): 1045-1047.

Yang Xiaoling;Cheng Zhou;Li Shan;Kitajima Akira;Hasegawa Kojiro. Self-hybridized Triploids Derived from 2n Gametes Induced by Colchicine inPummelo[J]. ACTA HORTICULTURAE SINICA, 2006, 33(5): 1045-1047.

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