Picloram, ABA和TDZ对香蕉体细胞胚胎发生的影响

园艺学报 ›› 2007, Vol. 34 ›› Issue (1): 81-86.

Picloram, ABA和TDZ对香蕉体细胞胚胎发生的影响

魏岳荣^1,2 ;杨　护¹ ; 黄秉智¹ ; 黄　霞² ;黄学林² ;邱继水¹ ;许林兵¹

(¹ 广东省农业科学院果树研究所, 广州510640; ² 中山大学生命科学学院教育部基因工程重点实验室, 广州510275)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-02-25 发布日期:2007-02-25

Effects of Picloram, ABA and TDZ on Somatic Embryogenesis of Banana

WEI Yue-rong^1,2; YANG Hu¹ ;HUANG Bing-zhi¹ ;HUANG Xia² ;HUANG Xue-lin² ;QIU Ji-shui¹ ;
XU Lin-bing¹

(¹ Fruit Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; ² The Key Laboratory ofGene Engineering of Ministry of Education, School of Life Sciences, Zhongshan University, Guangzhou 510275, China)

Received:1900-01-01 Revised:1900-01-01 Online:2007-02-25 Published:2007-02-25

摘要/Abstract

摘要： 研究Picloram、ABA和TDZ对贡蕉未成熟花序来源的胚性细胞悬浮系体细胞胚胎发生的影响。
结果表明, 8.28 μmol/L Picloram替代2, 4-D可获得15.56%的胚性愈伤组织诱导率; 在体细胞胚诱导初期
加入ABA则抑制体细胞胚的发生, 并造成其萌发时严重愈伤化; TDZ可有效地改善体胚萌发率和植株转换
率, 其中0.2 μmol/L TDZ处理的体胚萌发率和植株转换率分别从对照的17.28%、16.49%提高到72.77%
和67.05%。

关键词: 香蕉, 体细胞胚胎, Picloram, ABA, TDZ

Abstract: Establishment of a stable embryogenic cell suspension ( ECS) is a prerequisite for biotechno
logical breeding of banana. Although many important p rogress has been made, but app lication of embryogenic
cell suspension for genetic imp rovement ofM usa is limited because of low induction percentage of embryogeno
ic callus, low conversion frequence of p lant regeneration from the somatic embryos. Therefore, an op timal pro
tocol for embryogenic cell suspensions is still required to establish. In this study the effects of Picloram, ABA
and TDZ on somatic embryogenesis of banana from youngmale flower of Musa acuminata cv. Mas (AA) were
investigated. Results showed that the induction percentage of embryogenic calli reached 15.6% when 2, 4-D
in the callus induction medium was substituted by 8.28 μmol/L picloram. However, the addition ofABA into
somatic embryo development medium inhibited somatic embryogenesis from embryogenic cell suspension and
resulted in callus formation from somatic embryos. Treatmentwith TDZ at concentration of 0.2 μmol/L resul
ted in increase of the percentage of germination of somatic embryos from 17.28% to 72.77% and the plant
conversion ratio from 16.49% to 67.05% , respectively.

Key words: Banana, Somatic embryogenesis, Picloram, ABA, TDZ

魏岳荣;;杨　护;黄秉智;黄　霞;黄学林;邱继水;许林兵. Picloram, ABA和TDZ对香蕉体细胞胚胎发生的影响[J]. 园艺学报, 2007, 34(1): 81-86.

WEI Yue-rong;YANG Hu;HUANG Bing-zhi;HUANG Xia;HUANG Xue-lin;QIU Ji-shui;XU Lin-bing. Effects of Picloram, ABA and TDZ on Somatic Embryogenesis of Banana[J]. ACTA HORTICULTURAE SINICA, 2007, 34(1): 81-86.

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