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园艺学报 ›› 2007, Vol. 34 ›› Issue (1): 71-74.

• 果树 • 上一篇    下一篇

柑橘种间六倍体体细胞杂种开花及其花粉育性

郭文武*;陈春丽; 邓于洋;邓秀新   

  1. (华中农业大学作物遗传改良国家重点实验室, 武汉430070)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-02-25 发布日期:2007-02-25

Flowering and Pollen Fertility of an Interspecific CitrusHexaploid Somatic Hybrid

GUO Wen-wu
*;CHEN Chun-li; DENG Yu-yang; DENG Xiu-xin
  

  1. (National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-02-25 Published:2007-02-25

摘要: 二倍体锦橙+种间四倍体体细胞杂种HR (哈姆林甜橙+ 粗柠檬) 的种间六倍体体细胞杂种
植株移入温室7年后(即2004年) , 有1株六倍体首次开花, 花粉染色活力40.8% , 花粉萌发率3.7%;
2006年该植株再次开花, 花粉染色活力为20.3%。由于自然界中没有柑橘六倍体, 本研究是人工的方法创
造出可育的柑橘六倍体体细胞杂种植株的首例报道。

关键词: 柑橘, 六倍体, 体细胞杂种, 开花, 育性评价

Abstract: Interspecific hexap loid somatic hybrid between J incheng orange and HR
(Hamlin orange + Rough lemon ) regenerated via electrofusion flowered seven years after
transp lantation in greenhouse. Flow cytometry analysis further confirmed its true hexap loid
nature. Its pollen stainability and germinabilitywas 40.8% and 3.7% , respectively. In 2006,
the hexalpoid plant flowered again and its pollen stainability was 20.3%. Since there is no
natural citrus hexaploid plant, this is the first report of fertile hexap loid somatic hybrid plant in
Citrus.

Key words: Citrus, Hexaploid, Somatic hybrid, Flowering, Fertility evaluation