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园艺学报 ›› 2007, Vol. 34 ›› Issue (1): 75-80.

• 果树 • 上一篇    下一篇

45S rDNA在不同倍性沙田柚染色体上的荧光原位杂交分析

向素琼1,2 ; 汪卫星1 ; 梁国鲁1*   

  1. (1 西南大学园艺园林学院, 重庆400716; 2 中国农业科学院柑橘研究所, 重庆400712)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-02-25 发布日期:2007-02-25

Chromosome Localization of 45S rDNA in Different Ploidy Citrus grandis byFluorescence in situ Hybridization

XIANG Su-qiong1,2; WANG Wei-xing1 ;LIANG Guo-lu1*   

  1. (1 Horticulture and Landscape College, Southwest University, Chongqing 400716, China; 2Citrus Research Institute, ChineseAcademy of Agricultural Sciences, Chongqing 400712,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-02-25 Published:2007-02-25

摘要: 应用荧光原位杂交技术进行45S rDNA在不同倍性沙田柚中期染色体上的定位研究。结果表
明: 不同材料的NOR位点表现出明显的多态性和杂合性, 即二倍体具4个45S rDNA位点, 四倍体单株t1
和t2 则分别具有10个和8个位点。位点在染色体上的位置包括短臂末端、着丝粒区域、着丝粒至短臂区
域、着丝粒至长臂区域、长臂末端共5种不同类型。不同材料中包含的位置类型不完全相同。不同位置类
型的位点数也不相同, 以着丝粒及着丝粒至短臂区域的类型位点数最多, 其次是短臂末端。同源四倍体和
相应的二倍体相比, 出现了DNA水平的变异。对用于荧光原位杂交的柑橘染色体制片方法和rDNA在染色
体上的分布位置、同源四倍体和相应二倍体的遗传差异分析、重复序列的染色体原位杂交用于染色体识别
和物种演化研究等进行了讨论。

关键词: 沙田柚, 染色体, 倍性, 荧光原位杂交( FISH)

Abstract: Chromosome localization of 45S rDNA was studied bymeans of fluorescence in situ hybridiza
tion ( FISH) on metaphase chromosomes of different p loidy Citrus grandis. NOR loci of different materials
showed obvious polymorphism and heterozygosity. There were four 45S rDNA loci in diploid, while there were
ten loci in tetrap loid t1 and eight loci in tetrap loid t2. Chromosomal location of rDNA loci included five types,
i1e. the telomeric region of the short arm, centromere to the short arm region, the centromeric region, centro
mere to the long arm region, the telomeric region of the long arm. Different materials had incomp lete same
rDNA loci types. The number of loci was not the same in different situations, the most were types of centro
mere to short arm region and centromeric region, next was the telomeric region of the short arm. There was a
variation in DNA among the homologous tetrap loids with the corresponding dip loid. Meanwhile, the citrus
chromosome slides used for F ISH and rDNA loci situation on chromosome, genetic variation analysis among the
homologous tetrap loids and dip loids, repeated sequence of chromosome in situ hybridization (CISH) for chro
mosome identification and species evolvementwere discussed.

Key words: Citrus grandis, Chromosome, Ploidy, Fluorescence in situ hybridization ( FISH)