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园艺学报 ›› 2006, Vol. 33 ›› Issue (5): 957-962.

• 研究论文 • 上一篇    下一篇

早熟油桃子叶和胚轴离体培养再生植株的研究

韩明玉;张满让;田增胜;孙跃峰;田玉命;王安柱;赵彩平   

  1. (西北农林科技大学园艺学院, 陕西杨凌712100)
  • 收稿日期:2005-11-08 修回日期:2006-06-01 出版日期:2006-10-25 发布日期:2006-10-25

Plant Regenera tion with in Vitro Culture from Cotyledons and Hypocotyls ofEarly-ripen Nectarines (Prunus persica var. nectarina)

Han Mingyu; Zhang Manrang;Tian Zengsheng;Sun Yuefeng;Tian Yuming;Wang Anzhu;Zhao Caiping   

  1. (College of Horticulture, Northwest A & F University, Yangling, Shaanxi 712100, China)
  • Received:2005-11-08 Revised:2006-06-01 Online:2006-10-25 Published:2006-10-25

摘要: 以早熟油桃‘华光’和‘曙光’为试材, 对影响其子叶和胚轴再生的植物生长调节剂浓度及组合、培养基、胚发育时期等因素进行了研究。结果表明: 子叶培养中, 华光花后60 d为最佳取材时期,其子叶在MS + TDZ 2 mg/L +NAA 0.04 mg/L的培养基上再生率为75%; 曙光则要取花后68 d成熟期子叶,其在MS +BA 8 mg/L +NAA 0.04 mg/L培养基上再生率为66.7%。胚轴培养中, 上、下胚轴无显著差异,华光胚轴再生以G + TDZ 3.0 mg/L + KT 1.0 mg/L +NAA 3.0 mg/L 效果较好; 曙光胚轴再生以QL + TDZ2.0 mg/L +NAA 0.5 mg/L效果较好。

关键词: 油桃, 胚轴, 子叶, 再生植株

Abstract: The cotyledons and hypocotyls of early-ripen nectarine varieties, ‘Huaguang’ and ‘Shuguang’, were cultured in order to study the factors affecting their regeneration, such as density and combination of plant growth moderators, culture medium and embryo developing phases. Cotyledons of different development stages, namely 30, 40, 50, 60 days after blossom and mature cotyledons respectively, was cultured with MS medium mixed with BA, TDZ, ZT, KT and NAA. The results showed that the best time to culture cotyledons of‘Huaguang’was 60 days after blossom and the regeneration rate of the cotyledons was 75% when they were cultured with MS + TDZ 2 mg/L +NAA 0.04 mg/L. Mature cotyledons, 68 days after blossom, were the best for‘Shuguang’and the regeneration rate was 6617% when the cotyledons were cultured with MS +BA 8 mg/L +NAA 0.04 mg/L. There was no remarkable difference between upper and lower hypocotyls in their culture. The Gmedium combined with TDZ 3.0 mg/L, KT 1.0 mg/L, and NAA 3.0 mg/L was the best for Huaguang's hypocotyls regeneration. The QL medium combined with TDZ 2.0 mg/L and NAA 0.5 mg/L was the best for Shuguang's hypocotyls regeneration. The hypocotyls could directly regenerate plants.

Key words: Nectarine, Hypocotyl, Cotyledon, Plant regeneration