https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2009, Vol. 36 ›› Issue (9): 1249-1254.

• 果树 •    下一篇

‘国光’苹果及其红色芽变花青苷合成与相关酶活性的研究

刘晓静1,2;冯宝春3;冯守千1;王海波1;石俊1;王娜1;陈为一4;陈学森1*   

  1. (1 山东农业大学作物生物学国家重点实验室, 山东泰安271018; 2 山东省烟台市福山区农业局, 山东烟台265500;3 山东省潍坊市林业局, 山东潍坊261041; 4 山东省五莲县林业局, 山东日照276800)
  • 收稿日期:2009-03-26 修回日期:2009-07-13 出版日期:2009-09-25 发布日期:2009-09-25
  • 通讯作者: 陈学森

Studies on Anthocyanin Biosynthesis and Activities of Related Enzymes of‘Ralls’and Its BudMutation

LIU Xiao-jing1,2 ;FENG Bao-chun3;FENG Shou-qian1;WANG Hai-bo1;SHI Jun1; WANG Na1;CHEN Wei-yi4;CHEN Xue-sen1*   

  1. (1State Key Laboratory of Crop Biology, Shandong Agricultural University, Taipan, Shandong 271018, China; 2Yantai Agricultural Bureau, Yantai, Shandong 265500, China; 3Weifang Forest B ureau, W eifang, Shandong 261041, China; 4ulian Forest Bureau, Rizhao, Shandong 276800, China)
  • Received:2009-03-26 Revised:2009-07-13 Online:2009-09-25 Published:2009-09-25
  • Contact: CHEN Xue-sen

摘要: 以‘国光’苹果及其红色芽变为试材, 测定了果实发育期间的花青苷含量及其相关酶活性,并研究了套袋对芽变花青苷合成的影响。结果显示: ①在果实发育期间, 芽变果皮的花青苷含量明显高于‘国光’, 尤其是成熟期芽变果皮花青苷含量为132170 U·g-1FW, 而‘国光’仅为49140 U·g-1FW; ②在果实发育期间, 两个品种间PAL 和UFGT的酶活性无明显差异, 但芽变的CHI和DFR酶活性明显高于‘国光’, 表明芽变花青苷合成能力的提高与CHI和DFR酶活性高有关; ③套袋抑制芽变果皮花青苷的合成, 但解袋后花青苷的含量极显著升高, 解袋后4种酶的变化趋势差异较大, CHI和UFGT活性均迅速升高, 明显高于对照, 这与解袋后花青苷迅速合成相吻合。综上结果, 芽变与原有品种在着色机理上的关键指标是果皮花青苷含量和CHI酶活性。

关键词: 苹果, 芽变, 着色机理, 套袋

Abstract: Anthocyanin content and activities of related enzymeswere measured in‘Ralls’and its budmutation. Effect of bagging on anthocyanin biosynthesis in bud mutation was also studied. The results showed: ①Anthocyanin content in mature fruits was 132170 U·g-1FW in bud mutation which was very significantly higher than that in‘Ralls’(49140 U·g-1FW) ; ②Activities of PAL and UFGT were almost the same between the two materials, but CH I and DFR activitieswere significantly high in bud mutation, which were related to the increase of anthocyanin levels in the mutant; ③Bagging treatment inhibited anthocyanin synthesis of bud mutation. But anthocyanin content had significantly increased after removing the bags. Activities of CHI and UFGT increased rap idly and were much higher than those in the control after the bag removal. The increase was consistentwith the imp rovement of anthocyanin levels. Above all, the key index of colouration mechanism of‘Ralls’and bud mutation is anthocyanin content and CHI activity.

Key words: apple, bud mutation, coloring mechanism, bagging

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