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园艺学报 ›› 2021, Vol. 48 ›› Issue (7): 1386-1396.doi: 10.16420/j.issn.0513-353x.2021-0152

• 研究论文 • 上一篇    下一篇

西瓜果实形状的分子精准鉴定

李娜, 尚建立, 李楠楠, 周丹, 孔胜楠, 王吉明, 马双武*()   

  1. 中国农业科学院郑州果树研究所瓜类种质改良研究中心,郑州 450009
  • 收稿日期:2021-02-05 修回日期:2021-04-25 出版日期:2021-07-25 发布日期:2021-08-10
  • 通讯作者: 马双武 E-mail:mashuangwu@caas.cn
  • 基金资助:
    国家重点研发计划项目(2016YFD0100204-26);国家农作物种质资源平台项目(NICGR2015-016);国家作物种质资源保护和利用专项(2014NWB038);中国农业科学院科技创新工程专项经费项目(CAAS-ASTIP-2016-ZFRI);国家现代农业产业技术体系建设专项资金项目(CARS-25)

Accurate Molecular Identification for Fruit Shape in Watermelon (Citrullus lanatus

LI Na, SHANG Jianli, LI Nannan, ZHOU Dan, KONG Shengnan, WANG Jiming, MA Shuangwu*()   

  1. The Laboratory of Melon Crops,Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,China
  • Received:2021-02-05 Revised:2021-04-25 Online:2021-07-25 Published:2021-08-10
  • Contact: MA Shuangwu E-mail:mashuangwu@caas.cn

摘要:

为了实现西瓜果实形状的分子精准鉴定,利用“国家西瓜甜瓜中期库”的资源和高通量测序平台,利用两个不同的杂交分离群体(F2和BC1P1)分别鉴定到1个159 bp插入缺失和1个非同义SNP导致的果形突变(由圆果形变为长果形),且SNP突变在159 bp插入缺失的基因组区域内。利用这个SNP开发CAPS标记Markersun在两个群体和128份西瓜种质中进行分析,发现标记Markersun在两个群体中与果形表型共分离;在西瓜种质中可同时区分插入缺失和SNP的变异,且与果形表型共分离。同时这两个变异与果形的共分离在196份西瓜核心种质的基因型分型中也得到验证。此外,通过对不同类型西瓜种质的基因型分析发现,SNP变异导致的长果形出现的时间更早且独立遗传,而159 bp插入缺失引起的长果形是栽培西瓜驯化过程中产生的。本研究中首次利用不同类型的西瓜核心种质实现了西瓜果形的分子精准鉴定,还挖掘到两个西瓜果形的功能变异并开发标记,为西瓜果形的分子精准鉴定提供技术支撑,同时为果形性状基因功能验证提供靶标,加速了西瓜果形性状基因的调控机理研究。

关键词: 西瓜, 果形, 功能性分子标记, 核心种质, 精准鉴定

Abstract:

To realize the accurate molecular identification for fruit shape,we performed following study. Based on resource advantage of“National mid-term gene-bank for watermelon and melon”and re-sequencing platform,two segregation populations,including an F2 and an BC1P1,has been constructed to unravel the functional genetic variation for fruit shape in watermelon. A 159 bp InDel(Insertion/Deletion)and one non-synonymous SNP led round fruit to long fruit in watermelon,respectively. Moreover,the SNP located on the genomic region of 159 bp InDel. One CAPS marker,Markersun,was developed and genotyped in F2 and BC1P1 populations,and a core germplasm resources including 128 inbred lines. The marker Markersun showed InDel and SNP polymorphism in F2 and BC1P1 populations,respectively,and totally cosegregated with fruit shape variation in both populations. The marker Markersun both showed InDel and SNP polymorphism in core germplasm resources and totally cosegregated with fruit shape variation. The results were reconfirmed based on resequencing data of 196 germplasm resources in watermelon. Moreover,genotype analysis of different type of germplasms found that long fruit caused by SNP occurred earlier and inherited independently;while long fruit caused by 159 bp InDel occurred during domestication of cultivated watermelon. The current research firstly realized accurate molecular identification of watermelon fruit shape. At the same time,two functional variations of fruit shape were excavated and corresponding marker was developed to accurately identify watermelon fruit shape. These results will provide technical support and targets for the functional verification of fruit shape traits,and accelerate the study of the functional mechanism for fruit shape gene in watermelon.

Key words: Citrullus lanatus, fruit shape, functional molecular marker, core germplasm, accurate identification

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