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园艺学报 ›› 2021, Vol. 48 ›› Issue (3): 465-476.doi: 10.16420/j.issn.0513-353x.2020-0468

• 研究论文 • 上一篇    下一篇

欧洲葡萄VvMYB6正向调控花青素合成

朱自果1, 张庆田1, 韩真1, 李勃1, 李国栋2, 李秀杰1,*()   

  1. 1山东省果树研究所,山东泰安 271000
    2山东省果茶技术推广站,济南 250013
  • 收稿日期:2020-11-24 出版日期:2021-03-25 发布日期:2021-04-02
  • 通讯作者: 李秀杰 E-mail:lixiujie-2007@163.com
  • 基金资助:
    山东省自然科学基金项目(ZR2020MC137);山东省重大科技创新工程项目(2019JZZY010727);山东省重大科技创新工程项目(2018CXGC0208)

VvMYB6,an R2R3-MYB Transcription Factor,is Involved in Anthocyanin Biosynthesis of Grapevine

ZHU Ziguo1, ZHANG Qingtian1, HAN Zhen1, LI Bo1, LI Guodong2, LI Xiujie1,*()   

  1. 1Shandong Institute of Pomology,Tai'an,Shandong 271000,China
    2Shandong Fruit & Tea Technology Services,Ji’nan 250013,China
  • Received:2020-11-24 Online:2021-03-25 Published:2021-04-02
  • Contact: LI Xiujie E-mail:lixiujie-2007@163.com

摘要:

从欧洲葡萄‘粉红亚都蜜’中克隆到1个MYB转录因子基因VvMYB6。VvMYB6蛋白定位在细胞核,其N端包含1个保守的R2R3结构域。在葡萄中,VvMYB6主要在在根、花器官及果实发育早期表达。在烟草中异位表达VvMYB6,显著促进花瓣和雄蕊中花青素的积累;代谢组分析发现,相比野生型,转基因植株花中累积高含量的飞燕草色素和矢车菊色素。转基因烟草植株中查尔酮合酶(CHS)、查尔酮异构酶(CHI)、黄烷酮4-还原酶(DFR)、花青素合酶(ANS)和UDP葡萄糖-类黄酮-O-葡萄糖基转移酶(UFGT)基因表达显著上调。结果表明VvMYB6正向调控花青素合成。

关键词: 葡萄, MYB转录因子, 花青素

Abstract:

Here,we demonstrated that VvMYB6,an R2R3-MYB transcription factor,may function as an activator of anthocyanin biosynthesis in grapevine(Vitis vinifera L.). The VvMYB6 protein contains a conserved R2R3 domain at its N-terminal and localized to the nucleus. Expression studies in grapevine showed that VvMYB6 were strongly expressed in roots,flowers,and berry development of early stages. Ectopic expression of VvMYB6 in tobacco caused the accumulation of anthocyanins in petals and stamens. Metabolomics analysis showed that VvMYB6 expression also caused a marked accumulation of several phenolic compounds,including delphinidin and cyanidin. Expression analysis revealed that several anthocyanin biosynthesis structural genes,including chalcone synthease(CHS),chalcone isomerase(CHI),dihydroflavonol 4-reductase(DFR),anthocyanidin synthase(ANS)and UDP glucose flavonoid 3-O-glucosyl transferase(UFGT),were strongly up-regulated in transgenic plants. These findings suggest that VvMYB6 plays a key role in anthocyanin biosynthesis.

Key words: grapevine, MYB transcription factor, anthocyanin

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