关键词: 桃, 乙烯应答因子, 亚细胞定位, 功能分析

Abstract: In this study，an ethylene response factor was cloned from‘Fengguang’nectarine and named as PpERF1a（ppa018178m）. The full length of PpERF1a was 600 bp，which encoded 199 amino acids. PpERF1a contained a single AP2 domain, which was consistent with the ERF families’ features. Subcellular localization assays showed that PpERF1a was targeted to nucleus and cell membrane. Fifteen transgenic Arabidopsis plants overexpressing PpERF1a were generated. Comparing with the wild type Arabidopsis plants，transgenic plants showed obvious stunted phenotype. Six transgenic plants kept on producing rosette leaves without bolting or producing seeds. Six transgenic plants bolted and flowered，but no seed. Three transgenic plants could flower and produce seed，but the seed yield of the transgenic plants was obviously lower than that of the wild type. These results showed that PpERF1a gene regulated the growth and development of transgenic Arabidopsis plants. This study provides a technical basis for further analysis the functions of PpERF1a gene in peach growth and development．

Key words: peach, ethylene response factor, subcellular localization, function analysis