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园艺学报 ›› 2020, Vol. 47 ›› Issue (3): 467-476.doi: 10.16420/j.issn.0513-353x.2019-0510

• 研究论文 • 上一篇    下一篇

不同光质照射下真姬菇的生长发育与光受体的响应表达

宋寒冰 1,2,李 勇 1,2,黄嘉华 1,2,段静怡 1,2,张祺锶 1,2,谢宝贵 2,陶永新 1,2,*   

  1. 1 福建农林大学园艺学院,福州 350002;2 福建农林大学菌物研究中心,福州 350002
  • 出版日期:2020-03-25 发布日期:2020-03-25
  • 基金资助:
    福建农林大学园艺学院青年学术骨干培养基金项目(61201400706);福建农林大学科技创新专项基金项目(CXZX2017184);福建省种业创新与产业化工程项目(fjzycxny2017010);福建省食用菌产业体系项目(K83139296);福建省三区人才支持计划科技人员项目(K1519036A)

Growth and Development of Hypsizygus marmoreus and Response Expression of Light Receptor White Collar Genes Under Different Light Quality Irradiation

SONG Hanbing1,2,LI Yong1,2,HUANG Jiahua1,2,DUAN Jingyi1,2,ZHANG Qisi1,2,XIE Baogui2,and TAO Yongxin1,2,*   

  1. 1College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 350002,China;2Mycological Research Center of Fujian Agriculture and Forestry University,Fuzhou 350002,China
  • Online:2020-03-25 Published:2020-03-25

摘要: 采用 5 种不同光质光源,分别对真姬菇(Hypsizygus marmoreus)的菌丝、原基和子实体进行照射,检测菌丝生长速度、原基形成数量、子实体菌柄长度、菌柄直径、菌盖直径以及产量等。结果显示:真姬菇菌丝在黑暗和红光下生长较快,在红绿蓝复合光下生长最慢;在蓝光下形成的原基数量最多,而在红光下原基数量最少;子实体阶段,黑暗下菌柄的长度及直径最大,绿光下菌柄长度及直径最小;而在蓝光和红绿蓝复合光下菌盖直径最大。相同培养时间,真姬菇在黑暗和蓝光下产量较高,在绿光下产量最低。进一步检测真姬菇光受体基因 WC1 和 WC2 在不同光质条件下的差异表达。结果表明,在菌柄中 WC1 在黑暗下表达量最高,WC2 在蓝光下表达量最高;而在菌盖中 WC1 和 WC2 均在蓝光下表达量最高。

关键词: 真姬菇, 光照, 菌丝生长, 形态建成, 光受体

Abstract: It this study,the lights with five different waves were used to illuminate the mycelia,primordia and fruiting body of Hypsizygus marmoreus,respectively and the growth rate of the hypha,the number of the primordia,the stipe length and diameter,the pileus diameter as well as the yield of the fruiting body of H. marmoreus were detected. The results showed that hypha of H. marmoreus grew faster under the darkness condition or the red light,and the slowest under the red-green-blue composite light. The number of primordia of H. marmoreus was the most under the blue light,while the least under the red light. For the fruiting body of H. marmoreus,the length and diameter of the stipe were the largest under the darkness condition,while the smallest under the green light. The diameter of the pileus was larger under the blue light or the red-green-blue composite light. Under the same cultivation time,the yield of H. marmoreus was the highest under the darkness or the blue light condition,while the lowest under the green light. Further,the differential expression of two photoreceptors WC1 and WC2 under the above different light conditions was also detected by qPCR. In the stipe,the WC1 gene exhibited the highest expression under the darkness condition,while the WC2 gene exhibited the highest under the blue light. In the pileus, both WC1 and WC2 showed the highest expression level under the blue light.

Key words: Hypsizygus marmoreus, illumination, hypha growth, morphogenesis, photoreceptor

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