关键词: 红芽芋, 茎尖脱毒, RT-PCR检测, 试管芋诱导, 植株再生

Abstract: In order to solve the low survival rate and high differentiation rate of virus-free seedling of red bud taro seedlings in the process of transplanting，in this study，with taking the most famous and high-quality local cultivars of Yanshan red bud taro（Colocasia esculenta）in Jiangxi Province as experimental material，the detoxification culture of taro stem tip，molecular detection of test-tube plantlets and microtuber induction were carried out. The results showed that seedlings in vitro could be induced from 0.2–1.0 mm taro buds inoculated in MS + 2.0 mg • L-1 6-BA + 0.5 mg • L-1 NAA + 3% sucrose medium，and RT-PCR molecular detection indicated that the seedlings induced from stem tip under 0.3 mm diameter in culture could not carry virus（DsMV）. The optimal medium for the induction of secondary proliferation of virus-free seedlings was MS + 3.0 mg • L-1 6-BA + 0.5 mg • L-1 NAA + 3% sucrose，the optimal medium for the induction of virus-free cormel was MS + 1.0 mg • L-1 6-BA + 0.5 mg • L-1 NAA + 8% sucrose，the optimal culture virus-free seedlings and taro corm conditions were 25 ℃，54 μmol • m-2 • s-1，14 h • d-1，the optimal substrates for transplanting and acclimation of was peat︰vermiculite（2︰1），and the survival rate was 97.71%. Compared with the contrast，the yield of virus-free taro increased 43.14% and there were significant differences in nutritional characters between them. The formation of virus-free cormel and the establishment of a plant regeneration system provide a new way for the rapid propagation of healthy seedlings of red bud taro.

Key words: red bud taro, detoxification of stem tip, RT-PCR detection, induction of in vitro taro, plant regeneration