关键词: 草莓, FaRGA1, 匍匐茎, 开花, 短缩茎, 木质化

Abstract: RGA is one of the DELLA protein that is the core component of inhibition of gibberellin-mediated response in the gibberellin signaling pathway，and plays an important role in plant growth and development. In this study，the physicochemical characteristics and secondary structure of FaRGA1 in cultivated strawberry（Fragaria × ananassa）were predicted and the conserved domain of FaRGA1 protein was analyzed. Phylogenetic tree analysis shows that FaRGA1 has high homology with FveRGA1 in woodland strawberry（F. vesca），and the two are orthologous genes. The RNAi vector of strawberry RGA1 gene was constructed，and 3 transgenic lines with FaRGA1 silencing were obtained from strawberry cultivar‘Jingyu’by Agrobacterium-mediated genetic transformation. Compared with non-transgenic control plants，all three FaRGA1 silencing lines showed the characteristics of without flowers and continuously producing runners during two years，and the shortened crowns were significantly elongated and lignified. This study found that FaRGA1 gene has an inhibitory effect on tunner formation and promotes flowering in cultivated strawberries，which lays the foundation for molecular breeding of strawberry based on FaRGA1 gene in the future.

Key words: strawberry, FaRGA1, runner, flowering, crown, lignification