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园艺学报 ›› 2020, Vol. 47 ›› Issue (1): 23-40.doi: 10.16420/j.issn.0513-353x.2019-0581

• 研究论文 • 上一篇    下一篇

柑橘属SAUR基因家族的全基因组鉴定及表达分析

王福生1,2,余 洪1,胡 洲1,管德龙3,张 盼1,朱世平1,2,赵晓春1,2,*   

  1. 1西南大学/中国农业科学院柑桔研究所,重庆400712;2国家柑桔工程技术研究中心,重庆400712;3陕西师范大学生命科学学院,西安710119
  • 出版日期:2020-01-25 发布日期:2020-01-25
  • 基金资助:
    国家现代农业产业技术体系建设专项资金项目(CARS-26);重庆市现代山地特色高效农业技术体系创新团队建设计划项目;西南大学双一流学科建设项目;中国博士后科学基金项目(2017M622946);重庆市博士后科研项目(XmT2018062)

Genome-wide Analysis of SAUR Gene Family in Citrus

WANG Fusheng1,2,YU Hong1,HU Zhou1,GUAN Delong3,ZHANG Pan1,ZHU Shiping1,2,and ZHAO Xiaochun1,2,*   

  1. 1Citrus Research Institute,Southwest University/Chinese Academy of Agricultural Sciences,Chongqing 400712,China;2National Citrus Engineering Research Center,Chongqing 400712,China;3College of Life Sciences,Shaanxi Normal University,Xi’an 710119,China
  • Online:2020-01-25 Published:2020-01-25

摘要: 分析SAUR(Small auxin up-regulated RNA)基因家族在不同柑橘基因组中的数量、连锁群分布、基因结构、系统进化、基因表达模式等,从香橼、柚、莽山野橘和克里曼丁橘等基因组中分别鉴定到69、62、58和70个SAUR基因。SAUR在基因组上分布不均,呈现明显的串联重复现象,基因家族成员的差异可能是由于串联重复和染色体大片段复制引起。大多数柑橘SAUR基因不含内含子,含有SAUR特有的4个保守motif。系统进化树分析显示,来源于拟南芥、水稻、番茄、马铃薯和柑橘的629个SAUR基因分为9组,每一组都含有5个物种的家族成员,并且同一个物种的SAUR基因具有较近的遗传距离。顺式元件分析表明,CclSAUR基因家族成员的上游序列含有光响应、转录因子结合、激素响应、伤害响应、低温响应、玉米素代谢和类黄酮生物合成相关的元件。qRT-PCR分析结果显示,大多数CclSAUR都能响应外源IAA和低温处理。

关键词: 柑橘属, SAUR 基因家族, 基因表达, 全基因组鉴定

Abstract: Small auxin up-regulated RNA(SAUR)gene family plays a vital role in plant growth and development. In this study,a total of 70,69,58 and 62 SAUR homologous genes were identified from the genomes of Citrus clementina,C. medica,C. reticulata‘Mangshan’and C. maxima,respectively. Chromosomal location analysis revealed that the distribution of SAUR genes on chromosome is not uniform. Most of SAUR genes appeared as tandem duplication clustered on chromosome. The tandem duplication and segmental duplication could be the consequence of SAUR genes expansion. Most of citrus SAUR genes do not contain intron,and possess highly conserved four SAUR-specific motifs. The phylogenetic analysis displayed that 629 SAUR genes from 5 species were classified into 9 groups. In each group,the majority of species-specific SAUR genes have a close genetic relationship. cis-elements analysis revealed that light responsiveness,transcription factor(TF)binding,hormone responsiveness,wound responsiveness,low temperature responsiveness,zein metabolism and flavonoid biosynthesis related elements exist in upstream sequences of CclSAUR genes. The results of qRT-PCR analysis indicated that the expression of most of CclSAUR genes clearly responded to the exogenous IAA and cold temperature treatments. This study presented an overview information of constitution,structure and basic biological function of SAUR gene family in different citrus species.

Key words: Citrus, small auxin up-regulated RNA, gene expression, genome-wide analysis

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