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园艺学报 ›› 2019, Vol. 46 ›› Issue (2): 307-316.doi: 10.16420/j.issn.0513-353x.2018-0517

• 研究论文 • 上一篇    下一篇

大白菜抗TuMV显性位点F2的QTL-seq分析

李国亮,张淑江,钱 伟,李 菲,章时蕃,张 慧,方智远,孙日飞*   

  1. 中国农业科学院蔬菜花卉研究所,北京 100081
  • 出版日期:2019-02-25 发布日期:2019-02-25
  • 基金资助:

    国家重点研发计划项目(2016YFD0100204-06);国家自然科学基金项目(31772302);农业部园艺作物生物学与种质创制重点实验室项目

Genetic Analysis of Dominant Locus Involved in Resistance to Turnip mosaic virus by QTL-seq in Chinese Cabbage

LI Guoliang,ZHANG Shujiang,QIAN Wei,LI Fei,ZHANG Shifan,ZHANG Hui,FANG Zhiyuan,and SUN Rifei *   

  1. Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China
  • Online:2019-02-25 Published:2019-02-25

摘要:

为了挖掘大白菜抗TuMV基因的多样性,制定抗TuMV的遗传改良策略和综合防治措施,对抗TuMV的显性位点进行QTL分析。以大白菜对TuMV高抗的‘89B’和高感的‘强势’为亲本,构建F2群体。采用人工磨擦接种TuMV法对F2群体进行单株接种鉴定,经卡平方测验F2群体抗、感植株分离比例不符合3︰1(χ2 = 4.8 > χ0.052 = 3.84),非单基因遗传,为数量位点控制。选取表型高抗和高感的F2单株各40株进行混池,对2个极端池以及2个亲本进行重测序分析。通过计算抗、感池与亲本间的△(SNP-index),获得两个抗TuMV位点区域,物理位置为A07染色体的13.9 ~ 14.4 Mb和A08染色体的16.4 ~ 17.4 Mb。上述两个区域共筛选出具有多态性位点的基因68个,其中6个基因与植物抗性有关,其编号分别为Bra028499、Bra028500、Bra016311、Bra016312、Bra016313和Bra016314,其中Bra028499和Bra028500编码抗病蛋白,Bra016311、Bra016312、Bra016313和Bra016314编码抗TMV蛋白。Bra028499、Bra028500、Bra016311和Bra016314含有TIR-NBS-LRR特殊结构域。在已定位克隆的植物抗病基因中,大约80%属于NBS基因家族,因此推测这些基因可能与大白菜抗TuMV有关。

关键词: 大白菜, TuMV, 显性位点, QTL-seq, 遗传分析

Abstract:

In order to excavate the diversity of TuMV resistant genes and make the genetic improvement strategy and measures for the prevention and control in Chinese cabbage,QTL analysis of the dominant locus was conducted in the study,which would be helpful to cultivate the broad-spectrum resistance of Chinese cabbage varieties. The F2 population was constructed from the parents‘89B’(resistant)and‘Qiangshi’(susceptible),which were the highly inbred Chinese cabbage lines. The F2 population individuals were inoculated by TuMV C4 isolate,and the segregation from F2 did not fit the expected segregation for a Mendelian model 3︰1(χ2 = 4.8 > χ0.052 = 3.84),so quantitative trait locus controlled the character,not a single gene. Choosing 40 highly resistant/susceptible individuals from F2 population,respectively,to mix the pools,and the two parents and two pools were re-sequenced. By calculation analysis of △(SNP-index) between parent and pools,two main regions were obtained(A07 chromosome:13.9–14.4 Mb and A08 chromosome:16.4–17.4 Mb). There were 68 genes in the above two regions,and 6 out of 68 were associated with resistance in plants(Bra028499,Bra028500,Bra016311,Bra016312,Bra016313 and Bra016314). In addition,Bra028499 and Bra028500 coded the disease-resistant proteins,and Bra016311,Bra016312,Bra016313 and Bra016314 coded TMV-resistant proteins. Bra028499,Bra028500,Bra016311 and Bra016314 contained the TIR-NBS-LRR domains. About 80% of the resistance genes cloned in plants belonged to the NBS gene families,indicating that these genes may be associated with Chinese cabbage resistance to TuMV.

Key words: Chinese cabbage, TuMV, dominant locus, QTL-seq, genetic analysis

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