https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2019, Vol. 46 ›› Issue (11): 2073-2081.doi: 10.16420/j.issn.0513-353x.2018-1070

• 研究论文 •    下一篇

苹果MdNAC9的克隆及其调控黄酮醇合成功能的鉴定

孙庆国,姜生辉,房鸿成,张天亮,王 楠,陈学森*   

  1. 山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,山东泰安 271018
  • 出版日期:2019-11-25 发布日期:2019-11-25
  • 基金资助:
    国家自然科学基金项目(31730080,31701892);国家重点研究开发项目(2016YFC0501505)

Cloning of MdNAC9 and Functional of Its Regulation on Flavonol Synthesis

SUN Qingguo,JIANG Shenghui,FANG Hongcheng,ZHANG Tianliang,WANG Nan,and CHEN Xuesen*   

  1. College of Horticulture Science and Engineering,Shandong Agricultural University,State Key Laboratory of Crop Biology,Tai’an,Shandong 271018,China
  • Online:2019-11-25 Published:2019-11-25

摘要: 为了探究红肉苹果黄酮醇合成机理,分析通路相关基因,从成熟期红肉苹果中克隆了1个NAC转录因子基因MdNAC9。通过Real-Time PCR、酵母单杂交和荧光素酶报告试验,探究MdNAC9与苹果黄酮醇积累的相关性。结果表明,在苹果果实和过表达MdNAC9的愈伤组织(OENAC9)中MdNAC9表达量变化与黄酮醇合成相关基因MdFLS表达量变化趋势一致。酵母单杂交试验证明,MdNAC9具有转录激活功能,能够特异结合MdFLS基因启动子。荧光素酶报告试验显示,MdNAC9对MdFLS的启动子有激活作用,促进MdFLS的表达。MdNAC9可能通过激活MdFLS促进苹果黄酮醇积累。

关键词: 苹果, NAC转录因子, 酵母单杂交, 荧光素酶报告, 亚细胞定位, 黄酮醇

Abstract: In order to explore the mechanism of flavonol synthesis in red flesh apple,we analyzed the pathway-related genes using mature red flesh apples,and cloned a transcription factor gene MdNAC9. The correlation between MdNAC9 and flavonol accumulation was explored by Real-Time PCR,yeast one-hybrid and luciferase reporter assay. The results showed that the expression of MdNAC9 was positively correlated with MdFLS,a key gene in flavonol synthesis,in fruit and over-expressed callus (OENAC9). Yeast one-hybrid showed that MdNAC9 could specifically bind to MdFLS promoter. Luciferase reporter assay proved that MdNAC9 could promote the expression of MdFLS. MdNAC9 may promote flavonol accumulation by activating MdFLS.

Key words: apple, NAC transcription factor, yeast one-hybrid, luciferase reporter assay, subcellular localization, flavonol

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