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园艺学报 ›› 2018, Vol. 45 ›› Issue (12): 2277-2290.doi: 10.16420/j.issn.0513-353x.2018-0158

• 研究论文 •    下一篇

20个苹果品种的S基因型鉴定

丁体玉1,*,吴萌萌1,*,张瑞萍1,**,阎振立1,**,闫亚茹2,陈迪新2,张恒涛1,高启明1   

  1. 1中国农业科学院郑州果树研究所,郑州 450009;2河南科技大学,河南洛阳 471003
  • 出版日期:2018-12-25 发布日期:2018-12-25
  • 基金资助:
    国家自然科学青年基金项目(31401835);国家现代农业产业技术体系建设专项资金项目(CARS-28);中国农业科学院科技创新工程项目(CAAS-ASTIP-2016-RIP-02)

Identification of S-genotypes of 20 Apple Cultivars

DING Tiyu1,*,WU Mengmeng1,*,ZHANG Ruiping1,**,YAN Zhenli1,**,YAN Yaru2,CHEN Dixin2,ZHANG Hengtao1,and GAO Qiming1   

  1. 1Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,China;2Henan University of Science and Technology,Luoyang,Henan 471003,China
  • Online:2018-12-25 Published:2018-12-25

摘要: 以‘富士’、‘华瑞’、‘华硕’和‘华星’等20个苹果品种为材料,利用S等位基因高度保守氨基酸序列FTQQYQ和anti-1/MIWPNV设计的S基因通用引物,以及S等位基因多态性序列设计的19对特异引物,PCR扩增、测序以鉴定20个品种的S基因型;并用‘华瑞’和‘华硕’分别与‘美八’、‘锦秀红’、‘华冠’和‘富士’进行授粉试验验证S基因型的准确性。PCR结果表明:通用引物扩增S等位基因时,仅‘富士’、‘华瑞’、‘华硕’、‘华星’、‘美八’和‘红脆宝’6个品种有效地扩增出2条特异的S等位基因条带,其S基因型有S1S9、S9S24、S5S9和S5S24等4种;19对特异引物扩增S等位基因时,‘华帅’等14个品种扩增得到2条特异性条带,S基因型有S10S19、S2S3、S2S5、S3S10、S2S9、S5S24、S9S10、S3S10和S5S9等9种。因此,20个苹果品种的S基因型分别为:‘富士’S1S9,‘华瑞’和‘华硕’S9S24,‘华星’、‘美八’和‘红珍珠’S5S9,‘红脆宝’、‘华玉’和‘99-1-29’S5S24,‘华帅’S10S19,‘金玉’S2S3,‘早红’、‘华美’和‘嘎拉’S2S5,‘Seokwang’S3S10,‘锦秀红’、‘蜜玉’和‘华冠’S2S9,‘绿佳’S9S10,‘信浓红’S3S10。2015和2016年‘华瑞’与‘华硕’的正反交组合坐果率较低(低于15.52%);而‘华瑞’和‘华硕’分别与‘美八’、‘锦秀红’和‘华冠’、‘富士’品种的正反交组合坐果率较高(高于46.30%)。因此,本试验中相同S基因型的授粉组合其坐果率较低,不同S基因型的授粉组合其坐果率较高,授粉试验支持S基因型鉴定结果。

关键词: 苹果, 自交不亲和性, S基因型

Abstract: To identify 20 apple germplasms’ S-genotypes by PCR and sequencing,the universal primers and 19 pairs of specific primers of apple S-alleles were designed for‘Fuji’,‘Huarui’,‘Huashuo’,‘Huaxing’and so on,based on the highly conserved amino acid sequences FTQQYQ and anti-1/MIWPNV and the polymorphic sequences of S-alleles. To verify the accuracy of identified S-genotypes,reciprocal crosses-pollination experiments were carried out using‘Huarui’and ‘Huashuo’with‘Meiba’,‘Jinxiuhong’and‘Huaguan’,respectively. PCR results showed that when using the universal primers,two specific S-allele bands were amplified only from‘Fuji’,‘Huarui’,‘Huashuo’,‘Huaxing’,‘Meiba’and‘Hongcuibao’,whose S-genotypes were S1S9,S9S24,S5S9 and S5S24. When 19 pairs of specific primers were used for amplification,two specific bands were obtained from 14 cultivars,whose S-genotypes were S10S19,S2S3,S2S5,S3S10,S2S9,S5S24,S9S10,S3S10 and S5S9. Therefore,the S-genotypes for 20 apple cultivars were:S1S9 for‘Fuji’,S9S24 for‘Huarui’and ‘Huashuo’S5S9 for‘Huaxing’,‘Meiba’and‘Hongzhenzhu’,S5S24 for‘Hongcuibao’,‘Huayu’and‘99-1-29’,S10S19 for ‘Huashuai’,S2S3 for‘Jinyu’,S2S5 for‘Zaohong’,‘Huamei’and‘Gala’,S3S10 for‘Seokwang’,S2S9 for ‘Jinxiuhong’,‘Miyu’and‘Huaguan’,S9S10 for ‘Lüjia’,S3S10 for‘Shinano Red’,respectively. The pollination experiment showed that the fruiting rate of reciprocal crosses-pollination between‘Huarui’and ‘Huashuo’ germplasm was lower than 15.52% in 2015 and 2016. The fruit ratio of reciprocal crosses-pollination between‘Huarui’and‘Huashuo’with‘Meiba’,‘Jinxiuhong’,‘Huaguan’,‘Fuji’were higher than 46.30% in 2015 and 2016. Therefore,in this experiment,the same combination of S-genotypes had a low fruit setting percentage but a higher fruit setting percentage with different S-genotypes,indicating that the results of pollination supported the results of S-genotype identification.

Key words: Malus × domestica, self-incompatibility, S-genotype

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