https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2018, Vol. 45 ›› Issue (11): 2209-2216.doi: 10.16420/j.issn.0513-353x.2018-0297

• 研究报告 • 上一篇    下一篇

侵染广东辣椒的辣椒脉斑驳病毒的分子特征

汤亚飞1,2,裴 凡1,于 琳1,何自福1,2,*,佘小漫1,蓝国兵1,邓铭光1   

  1. (1广东省农业科学院植物保护研究所,广州 510640;2广东省植物保护新技术重点实验室,广州 510640)
  • 出版日期:2018-11-25 发布日期:2018-11-25

Molecular Characterization of Chilli veinal mottle virus Infecting Pepper in Guangdong Province

TANG Yafei1,2,PEI Fan1,YU Lin1,HE Zifu1,2,*,SHE Xiaoman1,LAN Guobing1,and DENG Mingguang1   

  1. (1Institute of Plant Protection,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China;2Guangdong Provincial Key Laboratory of High Technology for Plant Protection,Guangzhou 510640,China)
  • Online:2018-11-25 Published:2018-11-25

摘要:

辣椒脉斑驳病毒(Chilli veinal mottle virus,ChiVMV)是引起广东辣椒病毒病的主要病原之一,为了明确侵染为害广东辣椒的ChiVMV分子特征,采用分段RT-PCR和RACE扩增方法克隆了ChiVMV广东分离物(ChiVMV-GD)的基因组全序列,结果表明,除poly(A)尾外,ChiVMV-GD基因组大小为9 721 nt,编码一个大小350.44 kD多聚蛋白(位于167 ~ 9 436 nt),5′–非编码区(5′-UTR)和3′–非编码区(3′-UTR)分别含有166和285 nt,5′–末端结合病毒基因组连接蛋白(VPg),3′–末端含有poly(A)尾。序列同源性分析结果表明:ChiVMV-GD与ChiVMV其他分离物的基因组序列同源率为79.1% ~ 96.9%,其中与来自中国海南分离物(登录号:GQ981316.1)的同源率最高(96.9%)。系统进化分析结果显示,ChiVMV-GD与海南分离物聚集在一个小分支,说明与海南分离物亲缘关系最近。

关键词: 辣椒, 辣椒脉斑驳病毒, 广东分离物, 分子特征

Abstract:

Chilli veinal mottle virus(ChiVMV)is one of main pathogen causing pepper virus disease in Guangdong Province,China. To ascertain the molecular characterization of ChiVMV isolate Guangdong(ChiVMV-GD),the whole genome sequence of ChiVMV-GD was cloned by RT-PCR and RACE amplification. In addition to the poly(A)tail,the length of ChiVMV-GD genome was 9 721 nt and encoded a 350.44 kD polyprotein(coordinate 167–9 436 nt). The 5′-untranslated region(5′-UTR)and 3'-untranslated region(3′-UTR)contained 166 and 285 nt,respectively. The 5′-end contained a viral genome-linked protein(VPg). The 3′-end contained a poly(A)tail. The whole genome sequence of ChiVMV-GD shared 79.1% to 96.9% nucleotide sequence identities with other isolates of ChiVMV deposited in the GenBank database,with the highest nucleotide sequence identities to the isolate Hainan(GenBank accession number:GQ981316.1)at 96.9%. Phylogenetic analysis of ChiVMV-GD and the other 11 isolates of ChiVMV indicated that it clustered with the Hainan isolate to form a unique clade. This result showed that ChiVMV-GD had the closest relationship with ChiVMV Hainan isolate. This paper is the first report the molecular characterization of ChiVMV infecting pepper in Guangdong Province,China.

Key words: pepper, Chilli veinal mottle virus, Guangdong isolate, molecular characterization

中图分类号: