关键词: Gynura bicolor, transcriptome, sequencing, gene analysis, function annotation

Abstract:

Gynura bicolor is rich in anthocyanidin and other functional components. In order to explore genetic basis for the synthesis of these active substances，the transcriptome of Gynura bicolor was sequenced by Illumina HiSeq 2500 platform，a total of 21 387 624 reading fragments were generated which formed 33 314 unigenes by sequence splicing. 2 387 SSR were found in 7 792 unigenes over 1 kb. All unigenes were annotated by different databases. 22 048 and 14 417 unigenes were annotated against Nr and SwissProt database respectively，and 29 unigenes were related to anthocyanidin biosynthesis；by Pfam database the 13 909 unigenes were included in 5 198 families of protein functional regions and 12 unigenes were related to anthocyanidin biosynthesis；by GO database the 11 613 unigenes were divided into 3 categories containing 51 function groups and 24 unigenes were related to anthocyanidin biosynthesis；by COG and KOG databases the 6 589 and 13 498 unigenes were grouped into 24 functional categories and 25 ones，respectively；by KEGG database the 4 466 unigenes were divided into 108 metabolism pathways，47 unigenes involved flavonoid biosynthesis.

Key words: Gynura bicolor, transcriptome, sequencing, gene analysis, function annotation