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园艺学报 ›› 2015, Vol. 42 ›› Issue (8): 1591-1598.doi: 10.16420/j.issn.0513-353x.2015-0058

• 研究报告 • 上一篇    下一篇

番茄黄化曲叶病毒基因间区缺失突变体在北京的发现与证实

赵宇楠, 程晓龙, 赵萍萍, 陈亚萍, 芦佳, 李常保, 姚磊   

  1. 1沈阳工业大学电气工程学院,沈阳 110870;2北京市农林科学院农业生物技术研究中心,农业基因资源与生物技术北京市重点实验室,北京 100097;3北京市农林科学院蔬菜研究中心,北京 100097
  • 出版日期:2015-08-25 发布日期:2015-08-25
  • 基金资助:
    北京市农林科学院科技创新能力建设专项(KJCX20140202);北京市科技计划项目(D151100004515003);国家自然科学基金项目(31471881)

Identificate Intergenic Region Deletion of Tomato yellow leaf curl virus Isolated from Beijing

ZHAO Yu-Nan, CHENG Xiao-Long, ZHAO Ping-Ping, CHEN Ya-Ping, LU Jia, LI Chang-Bao, YAO Lei   

  1. 1School of Electrical Engineering,Shenyang University of Technology,Shenyang 110870,China;2Beijing Agro- Biotechnology Research Center,Beijing Academy of Agriculture and Forestry,Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology,Beijing 100097,China;3Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry,Beijing 100097,China
  • Online:2015-08-25 Published:2015-08-25

摘要: 在北京发现两种致病性有差异的番茄黄化曲叶病毒(Tomato yellow leaf curl virus,TYLCV)毒株,得到两个不同的TYLCV分离物BJ03和BJ04,并对它们进行了全长扩增测序。TYLCV-BJ03全长为2 781 bp,TYLCV-BJ04全长为2 740 bp。进化树分析显示,这两个毒株分别属于以色列株系TYLCV-IL进化分支下中国的两个不同亚群。二者核酸序列的同源性为99.15%,主要差异在基因间区(intergenic region,IR)。BJ04比BJ03的IR区在互补链方向TATA-box与保守的9核苷酸序列之间缺失41个碱基。通过针对缺失片段设计的特异引物验证,进一步证实了TYLCV的IR区缺失突变体的存在。在对温室中不同发病番茄样本的检测中,同时检测到被两种毒株单独侵染和复合侵染的植株。

关键词: 番茄, 番茄黄化曲叶病毒, 基因间区, 序列缺失

Abstract: Two distinct pathogenicity Tomato yellow leaf curl virus isolates from the greenhouse of Beijing were observed,termed BJ03 and BJ04 respectively. The full long genomic sequences of these two TYLCV isolates were sequenced;TYLCV-BJ03 is 2 781 bp,and TYLCV-BJ04 is 2 740 bp. The molecule evolution reveals that these two isolates belong to two different Chinese group of a recent evolution branch of Israel strain TYLCY-IL. They shared 99.15% nt identity. The main difference between these two isolates is the intergenic region(IR). The BJ04 isolate contained a 41-nt deletion in the intergenic region betweenthe TATA-box and the nonanucleotide motif. This IR deletion mutant of TYLCY was confirmed by specific primers designed for the deleted region. In the further inspections,the single infected and combine infected samples were detected.

Key words: tomato, Tomato yellow leaf curl virus, intergenic region, sequence deletion

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