https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2015, Vol. 42 ›› Issue (5): 890-896.doi: 10.16420/j.issn.0513-353x.2014-0951

• 蔬菜 • 上一篇    下一篇

黄瓜和黑籽南瓜幼苗根系边缘细胞对肉桂酸胁迫的应答差异

乔永旭   

  1. 唐山师范学院生命科学系,河北唐山 063000
  • 出版日期:2015-05-25 发布日期:2015-05-25
  • 基金资助:

    河北省科技计划项目(13226922);河北省高等学校科学技术研究青年基金项目(QN20132018);唐山师范学院博士基金项目(2014A04)

Studies on Different Response of Cinnamic Acid to Root Border Cells in Cucumber and Figleaf Gourd Seedlings

QIAO Yong-Xu   

  1. Department of Life Science,Tangshan Normal University,Tangshan,Hebei 063000,China
  • Online:2015-05-25 Published:2015-05-25

摘要: 为了探索肉桂酸(cinnamic acid,CA)对黄瓜和黑籽南瓜根系边缘细胞(root border cell,RBC)生理特性的影响,以其幼苗为试材,研究了0、0.125和0.250 mmol ? L-1 CA对两者RBC的数量、活率、黏胶层厚度、凋亡率以及幼苗根系活力的影响。结果表明:CA胁迫降低了黄瓜和黑籽南瓜RBC数、活率和根系活力,这种抑制效果在黄瓜上比在黑籽南瓜上更加明显;CA胁迫增加了两者RBC的黏胶层厚度和凋亡率,黑籽南瓜黏胶层厚度的增加量较黄瓜明显,RBC凋亡率的增加程度低于黄瓜。0.125 mmol ? L-1 CA对黄瓜和黑籽南瓜RBC的影响趋势与0.250 mmol ? L-1 CA一致,其影响程度低于后者。RBC附着根尖时,0.250 mmol ? L-1 CA处理的黄瓜的根系活力明显降低,为对照的83.3%,而黑籽南瓜则无显著变化。去除RBC后,0.250 mmol ? L-1 CA明显降低了黄瓜和黑籽南瓜的根系活力,且黄瓜的根系活力显著低于黑籽南瓜。RBC能增强植物抵御CA胁迫的能力;黑籽南瓜RBC因具有凋亡率低及较厚的黏胶层等特性,在抵御CA胁迫的能力方面强于黄瓜。

关键词: 黄瓜, 黑籽南瓜, 肉桂酸, 胁迫, 根系边缘细胞

Abstract: In order to study cinnamic acid(CA)stress on root border cells(RBCs)biological characteristic in cucumber and figleaf gourd seedlings,the number of RBCs,viability of RBCs,thickness of mucilage layer of RBCs,the apoptosis rate of RBC and root activities in both species seedlings stressed by 0,0.125,0.250 mmol ? L-1 CA were investigated. The results showed that the number of RBCs,viability of RBCs and root activities inhibited by CA in cucumber seedlings were higher than that in figleaf gourd seedlings. Thickness of mucilage layer of RBC increased was higher in CA treated figleaf gourd seedlings than that in CA treated cucumber seedlings,on the contrary apoptosis rate of RBCs was lower in CA treated figleaf gourd seedlings. For RBCs the same trend was observed in both species stressed by 0.125and 0.250 mmol ? L-1 CA,but the extent of influence by lower concentration CA was lesser than that by higher CA concentration. 0.250 mmol ? L-1 CA inhibited root activities,which were more serious in cucumber seedlings than in figleaf gourd seedlings. Removal of RBCs from root tips led to more severe decline of root activities in 0.250 mmol ? L-1 CA treated cucumber and figleaf gourd seedling,it was more obvious in root activity in cucumber seedlings. It was concluded that RBCs play an important role in protecting root from CA toxicity,especially in figleaf gourd seedlings because of the lower apoptosis rate of RBCs and more thickness of mucilage layer of RBCs.

Key words: cucumber, figleaf gourd, cinnamic acid, stress, root border cell

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