https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2015, Vol. 42 ›› Issue (2): 386-394.doi: 10.16420/j.issn.0513-353x.2014-0362

• 研究报告 • 上一篇    下一篇

广西野生濒危植物掌叶木遗传多样性的ISSR与SRAP分析

李雪萍,郭松,熊俊飞,郭华伟,郑琳,李在留   

  1. 1河南科技大学林学院,河南洛阳 471003;2南京林业大学江苏省林业生态工程重点实验室,南京 210037;3广西大学林学院,南宁 530004
  • 出版日期:2015-02-25 发布日期:2015-02-25
  • 基金资助:
    国家自然科学基金项目(31060053);河南省自然科学基金项目(62580021);广西自然科学基金项目(2011GXNSFB018016)

Genetic Diversity of the Endangered Handeliodendron bodinieri in Guangxi Province by ISSR and SRAP Analysis

LI Xue-ping1,GUO Song2,3,XIONG Jun-fei1,3,GUO Hua-wei1,ZHENG Lin1,and LI Zai-liu3,*   

  1. 1College of Forestry,Henan University of Science and Technology,Luoyang,Henan 471003,China;2Jiangsu Key Laboratory of Forestry Ecological Engineering,Nanjing Forestry University,Nanjing 210037,China;3College of Forestry,Guangxi University,Nanning 530004,China
  • Online:2015-02-25 Published:2015-02-25

摘要: 采用ISSR和SRAP技术对中国野生濒危植物掌叶木[Handeliodendron bodinieri(Lévl.)Rehd.]的5个野生居群的95份材料进行分析,10条ISSR引物共扩增得到114个条带,其中多态性条带68个,多态性条带百分率(PPB)59.65%,掌叶木在物种水平上的Nei’s基因多样性(H)为0.1817,Shannon’s遗传多样性信息指数(I)为0.2792;观测等位基因数(Na)为1.5965,有效等位基因数(Ne)为1.2984,居群间基因间分化度(Gst)为35.17%。15组SRAP引物共扩增出292个条带,其中多态性条带269个,多态性条带百分比为92.12%,物种水平上,掌叶木的Nei’s基因多样性(H)为0.3171,Shannon’s遗传多样性信息指数(I)为0.4758;观测等位基因数(Na)为1.9212,有效等位基因数(Ne)为1.5380,居群间基因间分化度(Gst)为23.17%。结果显示两种标记均能检测到掌叶木具有较高的遗传多样性,掌叶木不同居群间存在一定的遗传分化和基因流动,但遗传分化主要存在于居群内。

关键词: 掌叶木, ISSR, SRAP, 遗传多样性, 居群, 遗传分化

Abstract: Genetic diversity of 95 samples of five natural populations of the endangered plant Handeliodendron bodinieri(Lévl.)Rehd. was analyzed by using ISSR and SRAP techniques. A total of 114 bands were obtained with 10 ISSR primers,of which 68 bands were polymorphic with a polymorphic proportion of 59.65%. At the species level,the Nei’s genetic diversity(H)was 0.1817,and Shannon’s information index(I)was 0.2792. The observed number of alleles was 1.5965 and the effective number of alleles was 1.2984. The differentiation coefficient(Gst)among the populations was 35.17%. Totally,292 bands were obtained with 15 SRAP primer pairs,and 269 bands were polymorphic with a proportion of92.12%. The Nei’s genetic diversity(H)was 0.3171,and the Shannon’s information index(I)was 0.4758. The observed number of alleles was 1.9212 and the effective number of alleles was 1.5380. The differentiation coefficient(Gst)among the populations by SRAP analysis was 23.17%. The results showed that both methods could be used to determine the genetic diversities among different populations of H. bodinieri. There was a high genetic diversity in H. bodinieri,and genetic differentiation and gene flow existed between the populations,but the main genetic differentiation occurred largely within the population.

Key words: Handeliodendron bodinieri, ISSR, SRAP, genetic diversity, population, genetic differentiation

中图分类号: