https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
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https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2015, Vol. 42 ›› Issue (11): 2105-2112.doi: 10.16420/j.issn.0513-353x.2015-0288

• 果树 •    下一篇

苹果对炭疽菌叶枯病抗性遗传的研究及其分子标记筛选

刘源霞1,2,李保华3,王彩虹2,刘春晓2,孔祥华2,祝 军2,戴洪义2,*   

  1. 1湖南农业大学园艺园林学院,长沙 410128;2青岛农业大学园艺学院,青岛市园艺植物遗传改良与育种重点实验室,山东青岛 266109;3青岛农业大学农学与植物保护学院,山东青岛 266109
  • 出版日期:2015-11-25 发布日期:2015-11-25
  • 基金资助:

    国家现代农业产业技术体系建设专项资金项目(CARS-28-01-07);山东省科技发展计划项目(2014GNC110017);山东省良种产业化工程项目(620902);‘十二五’农村领域国家科技计划项目(2013BAD02B01)

Genetic Studies and Molecular Markers Screening of Apple Resistance to Glomerella Leaf Spot

LIU Yuan-xia1,2,LI Bao-hua3,WANG Cai-hong2,LIU Chun-xiao2,KONG Xiang-hua2,ZHU Jun2,and DAI Hong-yi2,*   

  1. 1Horticulture & Landscape College,Hunan Agricultural University,Changsha 410128,China;2Qingdao Key Laboratory of Genetic Improvement and Breeding in Horticultural Plants,College of Horticulture,Qingdao Agricultural University,Qingdao,Shandong 266109,China;3College of Agronomy and Crop Protection,Qingdao Agricultural University,Qingdao,Shandong 266109,China
  • Online:2015-11-25 Published:2015-11-25

摘要:

利用两个对炭疽菌叶枯病高抗的苹果品种(系)‘富士’和‘QF-2’及两个高感品种‘金冠’和‘嘎拉’为亲本配制了4个杂交群体‘富士’ב金冠’,‘金冠’ב富士’,‘嘎拉’ב富士’,‘富士’בQF-2’。以F1群体植株为试验材料,对苹果炭疽菌叶枯病的抗性进行鉴定评价和遗传分析。结果表明,4个群体中抗、感植株的分离比分别符合1︰1,1︰1,0︰1和1︰0的理论比值,初步推测苹果抗炭疽菌叶枯病性状受隐性单基因控制,抗病基因型为rr,感病基因型为RR和Rr。以‘金冠’ב富士’F1群体为试材,采用分离群体分组分析(BSA)方法,通过对均匀覆盖苹果染色体组的500对SSR引物的筛选,获得了一个与抗病性状相关的分子标记S0506206-24,该标记与抗性基因位点的连锁距离为9.8 cM。

关键词: 苹果, 炭疽菌叶枯病, 抗性遗传, SSR标记

Abstract:

Genetic study of apple resistance to Glomerella Leaf Spot(GLS)were carried out by using 4 F1 hybrid groups(‘Fuji’בGolden Delicious’,‘Golden Delicious’בFuji’,‘Gala’בFuji’and‘Fuji’בQF-2’)generated from two highly resistant variety or selection,‘Fuji’and‘QF-2’,and two highly susceptible varieties‘Golden Delicious’and‘Gala’. The results showed that the separation ratio of resistant plants to the susceptible ones in 4 F1 hybrid groups were statistically consistent with the theoretical ratios of 1︰1,1︰1,1︰0 and 0︰1. By comprehensive analysis,the conclusions could be drawn as the follows:The apple resistance to GLS may controlled by a single recessive gene. The genotype of the resistant plants was rr,while the genotypes of the susceptible ones were RR and Rr. Using‘GoldenDelicious’בFuji’F1 hybrid groups and the Bulked Segregation Analysis(BSA)method,the marker S0506206-243 bp associated with disease resistance character to GLS were identified through screening 500 SSR primers covering the whole apple genome evenly,and the genetic distance between the marker and the resistant gene to GLS was 9.8 cM.

Key words: apple, Glomerella leaf spot, inheritance of resistance, SSR marker

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