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园艺学报 ›› 2015, Vol. 42 ›› Issue (1): 38-46.doi: 10.16420/j.issn.0513-353x.2014-0694

• 蔬菜 • 上一篇    下一篇

棕果番茄果实颜色遗传及gf位点序列变异分析

肖良军,陆佳楠,李宁,刘小茜,杨文才   

  1. 中国农业大学蔬菜学系,设施蔬菜生长发育调控北京市重点实验室,北京 100193
  • 出版日期:2015-01-25 发布日期:2015-01-25
  • 基金资助:

    国家重点基础研究发展计划(‘973’计划)项目(2012CB113900)

Genetics of Fruit Color and Sequence Variation of gf Locus in Brown Tomato Lines

XIAO Liang-Jun, LU Jia-Nan, LI Ning, LIU Xiao-Qian, YANG Wen-Cai   

  1. Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops,Department of Vegetable Science,China Agricultural University,Beijing 100193,China
  • Online:2015-01-25 Published:2015-01-25

摘要: 将番茄红果材料‘OH 88119’与棕果材料‘Black Cherry’杂交获得F2分离群体。通过对成熟果实外果皮、中果皮/内果皮和胎座颜色的观察,在不考虑番茄红素颜色的情况下,外果皮和中果皮/内果皮的颜色分别由一个基因控制,且独立遗传。用gf位点特异引物经PCR和RT-PCR扩增和测序,获得了‘Black Cherry’和‘紫色圣女果’的基因组DNA序列以及8个棕果番茄的cDNA序列,与红果材料中对应的GF位点序列相比,‘Black Cherry’、‘黑番茄(9T)-h’、‘黑番茄–2012’和‘紫果(圆)’的编码区出现了一个T与C替换,‘紫果(椭圆)’编码区缺失了两个核苷酸AT,而‘紫色圣女果’、‘紫玉(F1)-h-h’和‘Chocolate Cherry’则在编码区插入了一个A,这些突变分别属于gf 4、gf 3和gf 2类型,都形成新的终止密码子。根据序列差异建立了可用于鉴定gf 3和gf 4的dCAPS(Derived Cleaved Amplified Polymorphic Sequence)标记,为棕果番茄的标记辅助选择提供工具。

关键词: 番茄, 棕果, gf基因, 序列变异

Abstract: Exocarp,mesocarp,endocarp,and placenta colors were recorded from ripe fruits of each individual in the F2 population derived from a cross between the red-fruited line OH 88119 and brown-fruited line Black Cherry. Without considering the color of lycopene,the exocarp color and mesocarp/endocarp color inherited independently with one gene each. Genomic DNA was amplified from tomato lines Black Cherry and Purple Cherry by PCR with gene-specific primers. cDNA was also obtained from 8 brown lines using RT-PCR with the same gene-specific primers. Comparing to the sequences of GF gene in red-fruited tomato line,a substitution of T/C occurred in coding regions of Black Cherry,Heifanqie(9T)-h,Heifanqie-2012 and Ziguo(Round),a deletion of AT occurred in coding region of Ziguo(Oval),and an insertion of A in coding regions of Purple Cherry,Ziyu(F1)-h-h and Chocolate Cherry. These mutants belonged to gf 4,gf 3 and gf 2,and formed novel stop codon. A Derived Cleaved Amplified Polymorphic Sequence(dCAPS)marker for identifying gf 3 and gf 4 was developed based on the sequencevariations,which will provide a tool for marker-assisted selection of brown fruit in tomato.

Key words: tomato, brown fruit, gf gene, sequence variation

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