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园艺学报 ›› 2015, Vol. 42 ›› Issue (1): 19-30.doi: 10.16420/j.issn.0513-353x.2014-0752

• 果树 • 上一篇    下一篇

基于ndhF序列的中国悬钩子属植物系统发育研究

张丽, 王燕, 陈清, 罗娅, 张勇, 汤浩茹, 王小蓉   

  1. 1四川农业大学园艺学院,四川雅安 625014;2四川农业大学果蔬研究所,成都 611130
  • 出版日期:2015-01-25 发布日期:2015-01-25
  • 基金资助:

    国家自然科学基金项目(31272134)

Phylogenetic Utility of Chinese Rubus(Rosaceae)Based on ndhF Sequence

ZHANG Li, WANG Yan, CHEN Qing, LUO Ya, ZHANG Yong, TANG Hao-Ru, WANG Xiao-Rong   

  1. 1College of Horticulture,Sichuan Agricultural University,Ya’an,Sichuan 625014,China;2Institute of Pomology and Olericulture,Sichuan Agricultural University,Chengdu 611130,China
  • Online:2015-01-25 Published:2015-01-25

摘要: 悬钩子属植物种类繁多,属内系统演化规律一直存在争议。以近缘的同为蔷薇科蔷薇亚科的蔷薇属黄刺玫(Rosa xanthina)和草莓属野草莓(Fragaria vesca)为外类群,基于ndhF序列利用最大简约法和贝叶斯推论法构建了中国悬钩子属下3组17亚组的53个种(变种)的系统发育树。结果表明:ndhF序列在本研究涉及到的分类群中较保守,序列比对后长度为1 182 bp,其中变异位点88个(占7.44%),信息位点47个(占3.97%)。空心莓组(section Idaeobatus)的序列变异速率较木莓组(sect. Malachobatus)和刺毛莓组(sect. Dalibardastrum)快。木莓组需要结合其他进化速率相对较快的序列进一步研究。系统树分析表明,木莓组和刺毛莓组聚为一支,空心莓组则被划分为4支(BI),呈多系进化现象。中国悬钩子属植物的系统演化路线为复叶类群到单叶类群,空心莓组较木莓组原始,支持陆玲娣等对悬钩子属的系统演化观点。本研究结果有助于优良树莓种质的鉴定和分类,也为悬钩子属系统进化积累了分子依据。

关键词: 悬钩子属, 空心莓组, 木莓组, ndhF序列, 系统发育

Abstract: Rubus is a large and taxonomically complex group that displays agamospermy,polyploidy and frequent hybridization. The phylogeny of the genus has long been controversial. It is also difficult to identify and classify the wild excellent Rubus germplasms. The chloroplast DNA fragments ndhF of 53 Rubus species(variety)were analyzed for phylogenetic relationships among the genus by the Maximum parsimony(MP)and Bayesian inference(BI)analysis. Rosa xanthina and Fragaria vesca were used as outgroups,which were related to Rubus and belonged to Rosoideae subfamily. The results revealed that the ndhF among the genus was relatively conserved and 1182 bp in length. There were 88(7.44%)variable sites and 47(3.97%)parsimony information sites. The sequence divergence in section Idaeobatus was faster than that in sections Malachobatus and Dalibardastrum. Multiple DNA regions evolved rapidly should be used to resolve phylogenetic relationships within sect. Malachobatus. The phylogenetic tree indicated that those species from sections Malachobatus and Dalibardastrum formed a well-supported clade,whilethe species of sect. Idaeobatus were divided into four clades and polyphyletic. Based on the data,it was inferred that evolution in Rubus proceeded from compound leaf to simple leaf,and sect. Idaeobatus were the primitive group. These results supported the evolution view of Lu. It is helpful to identify and classify the wild excellent Rubus germplasms,as well as accumulate molecular data for the phylogeny of Rubus.

Key words: Rubus, sect. Idaeobatus, sect. Malachobatus, ndhF, phylogenetic utility

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