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园艺学报 ›› 2014, Vol. 41 ›› Issue (5): 994-1000.

• 研究报告 • 上一篇    下一篇

蔷薇属 3 个野生种中45S rDNA 和5S rDNA 的物理定位

张 婷,蹇洪英,田 敏,王其刚,张 颢,晏慧君,邱显钦,唐开学*   

  1. 云南省农业科学院花卉研究所,云南省花卉育种重点实验室,国家观赏园艺工程技术研究中心,昆明 650205
  • 出版日期:2014-05-25 发布日期:2014-05-25
  • 基金资助:

    国家自然科学基金地区基金项目(31060267);云南省科技攻关项目(2011BB013)

Physical Location of 45S rDNA and 5S rDNA in the Genomes of Three Wild Rose Species

  1. Flower Research Institute,Yunnan Academy of Agricultural Sciences,Yunnan Key Laboratory for Flower Breeding,
    National Engineering Research Center for Ornamental Horticulture,Kunming 650205,China
  • Online:2014-05-25 Published:2014-05-25

摘要: 采用双色荧光原位杂交(FISH)技术对3 个二倍体蔷薇野生种:多苞蔷薇(Rosa multibracteata
Helm. et Wils.)、川滇蔷薇(R. soulieana Crép.)和金樱子(R. laevigata Michx.)的体细胞中期染色体进行
了45S rDNA 和5S rDNA 物理定位。结果表明:45S rDNA 在这3 种蔷薇的染色体上的数量和分布模式较
一致,都有1 对位点,均位于一对亚中部着丝点异形同源染色体的短臂上。5S rDNA 在多苞蔷薇上有1
对位点,在川滇蔷薇和金樱子上各有2 对位点,都分布于染色体长臂的近着丝点处。这3 种蔷薇各自的
rDNA 位点数量、所在染色体和信号强弱有明显差异。研究结果为识别3 种蔷薇各自的染色体提供了明确
有效的分子细胞遗传学标记。

关键词: 蔷薇属, rDNA, 荧光原位杂交(FISH), 核型

Abstract: 45S rDNA and 5S rDNA were mapped on the metaphase chromosomes of 3 diploid wild
rose species:Rosa multibracteata Helm. et Wils.,R. soulieana Crép. and R. laevigata Michx.,respectively,
by double color fluorescence in situ hybridization. The 45S rDNA chromosomal distribution patterns were
highly conserved among the three species. Each species had a pair of 45S rDNA loci on the short arms of
one pair of heteromorphic submetacentric homologous chromosomes. The 5S rDNA sites were different in
number and location among the three species. A pair of 5S rDNA signals was detected on the
chromosomes of R. multibracteata,and two pairs of 5S rDNA sites were detected on those of both R.
soulieana and R. laevigata. 5S rDNA were localized near the centromere region on the long arms of
chromosomes. The number,location and fluorescent intensity of the rDNA signals varied somewhat in
these three species. These results indicated that the double color FISH with 45S rDNA and 5S rDNA as
probes supplied some characteristic cytomolecular markers for identifying their own chromosomes for these species.

Key words: Rosa L., rDNA, fluorescence in situ hybridization(FISH), karyotype

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