关键词: 百子莲, FT基因, 克隆, 基因表达

Abstract: The full length sequence of cDNA，FT gene of Agapanthus praecox，which was named ApFT with the accession number of KC951108. The results of sequence analysis showed that the ApFT ，which contained an open reading frame of 534 bp，177 encoding amino acids，untranslated region（UTR）5′ and 3′ with the length of 89 bp and 192 bp，respectively. A single and conservative domain of phosphatidylethanolamine-binding protein（PEBP）was contained in the encoded proteins of ApFT，which highly similar with the FT proteins in species such as Zea mays（MFT2），Arabidopsis thaliana（AtFT）and Citrus unshiu（CuMFT1）with the homology of 50% or more. According to the phylogenetic analysis results，ApFTof A. praecox has the closest genetic relationship with clustering corn（MFT2）. The real-time quantitative PCR results showed that the expression of ApFT in leaf was obviously higher than that in shoot tip during the whole period of flower bud differentiation. The expression of ApFT in leaf was going down with the differentiation of flower bud. For the shoot tips，the expression of ApFT in induction period was higher than those in vegetative and bud differentiation periods，which is conjectured that ApFT may play a crucial role in regulating the differentiation of Agapanthus praecox buds.gene length of cDNA was 815 bp ssp. orientalis was gained by the RACE method

Key words: Agapanthus praecox ssp. orientalis, ApFT, clone, gene expression