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园艺学报 ›› 2014, Vol. 41 ›› Issue (11): 2188-2195.

• 果树 • 上一篇    下一篇

平邑甜茶MhSnRK1在番茄中超表达对植株碳代谢的影响

王贵芳,彭福田*,张亚飞,党祝庆,王娜娜   

  1. 山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,山东泰安 271018
  • 收稿日期:2013-05-15 出版日期:2014-11-25 发布日期:2014-11-25
  • 基金资助:

    国家现代农业产业技术体系建设专项资金项目(CARS-31-3-03);山东省高校优秀科研创新团队计划项目

Effects of Overexpressing Pingyi Tiancha MhSnRK1 on Carbohydrate Metabolism in Tomato

WANG Gui-Fang, PENG Fu-Tian-*, ZHANG Ya-Fei, DANG Zhu-Qing, WANG Na-Na   

  1. College of Horticulture Science and Engineering,Shandong Agricultural University,State Key Laboratory of Crop Biology,Tai’an,Shandong 271018,China
  • Received:2013-05-15 Online:2014-11-25 Published:2014-11-25

摘要: 以超表达平邑甜茶(Malus hupehensis Rehd. var. pingyiensis Jiang)SnRK1(蔗糖非发酵–1–型相关蛋白激酶–1)基因MhSnRK1的番茄株系O-7及野生型番茄(Solanum lycopersicum)为试材,研究MhSnRK1对番茄植株碳代谢的影响。结果表明,与野生型番茄相比,超表达MhSnRK1番茄数字基因表达谱分析显示,光合途径中差异表达的10个基因中7个基因转录被上调;功能叶片的净光合速率日变化平均值提高20.3%;嫩叶及功能叶SnRK1酶活性比野生型功能叶提高20.6%和25.0%,可溶性糖提高57.7%和27.0%;超表达MhSnRK1番茄嫩叶SnRK1酶活性明显高于功能叶,淀粉含量提高了近1倍,而野生型番茄嫩叶及功能叶差异不明显。海藻糖处理后,超表达MhSnRK1番茄和野生型番茄净光合速率都下降,且野生型下降幅度大于超表达MhSnRK1番茄;超表达MhSnRK1及野生型番茄嫩叶和功能叶SnRK1酶活性都降低,但超表达MhSnRK1番茄仍高于野生型。

关键词: 平邑甜茶, SnRK1, 番茄, 数字基因表达谱, 可溶性糖, 淀粉, 净光合速率

Abstract: MhSnRK1-overexpressing tomato(O-7)and wild type tomato were used to investigate the function of MhSnRK1 on carbohydrate metabolism. The results showed that ten genes in photosynthesis pathway were differentially expressed between MhSnRK1-overexpressing line and wild type,among which seven genes were up-regulated in MhSnRK1-overexpressing line through digital gene expression(DGE)analysis. Compared with wild type tomato,the average photosynthetic rates increased by 20.3% in mature leaves of MhSnRK1-overexpressing tomato. SnRK1 activities in young and mature leaves increased respectively by 20.6% and 25.0% of MhSnRK1-overexpressing tomato. The soluble sugar contents in young leaves and in mature leaves increased by 57.7% and 27.0% respectively. In young leaves of MhSnRK1-overexpressing tomato SnRK1 activity was obviously higher than that in mature leaves. Thestarch contents were nearly doubled in young leaves of MhSnRK1-overexpressing tomato,while there was no obvious difference in starch content between these leaves of the wild type. After trehalose treatments,the photosynthetic rates decreased in mature leaves of MhSnRK1-overexpressing and wild type tomato and wild type tomato decreased more. SnRK1 activities in young and mature leaves of MhSnRK1-over- expressing and wild type tomato both decreased,while MhSnRK1-overexpressing line was still higher than wild type tomato.

Key words: Malus hupehensis Rehd. var. pingyiensis Jiang, SnRK1, tomato, digital gene expression-tag profiling, soluble sugar, starch, photosynthetic rate

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