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园艺学报 ›› 2012, Vol. 39 ›› Issue (8): 1491-.

• 蔬菜 • 上一篇    下一篇

白菜型油菜和菜薹的InDel 标记开发及其RILs群体遗传连锁图谱的构建

 孟霖, 刘博, 林良斌, 程峰, 王晓武, 武剑   

  1. (1 云南农业大学农学与生物技术学院,昆明 650201;2 中国农业科学院蔬菜花卉研究所,北京 100081)
  • 出版日期:2012-08-25 发布日期:2012-08-25

Development of InDel Markers for Brassica campestris and Genetic Linkage Map Construction of the RILs Population

 MENG  Lin, LIU  Bo, LIN  Liang-Bin, CHENG  Feng, WANG  Xiao-Wu, WU  Jian   

  1. (1College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201,China;2Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China)
  • Online:2012-08-25 Published:2012-08-25

摘要: 通过白菜型油菜‘R-O-18’和菜薹‘L58’的基因组重测序数据与白菜基因组的参考序列(‘Chiifu-401-42’的基因组序列)的比对,在全基因组范围内检测到了18 479 个短插入缺失变异位点(≤5 bp)。从中挑选了500 个插入/缺失片段为4 ~ 5 bp 的InDel 变异位点,将其设计成InDel 分子标记并进行试验验证,结果有452 个标记通过PCR 扩增出单一条带,但仅有106 个在‘R-O-18’和‘L58’间表现出多态性,346 个没有多态性,48 个在PCR 中没有扩增。亲本间具有多态性的106 个InDel 标记可用来检测以‘R-O-18’和‘L58’为亲本构建的RILs 基因型,并构建了一张包含99 个标记的遗传连锁图谱。

关键词: 菜薹, 油菜, 白菜, 插入/缺失, 全基因组重测序, Illumina 测序, 遗传连锁图

Abstract: Genome-wide Brassica campestris short Insertion/Deletion length polymorphisms(InDels)(≤ 5 bp)were detected by aligning whole genome re-sequencing data from two B. campestris accessions,‘R-O-18’and‘L58’,to the reference genome sequence of‘Chiifu-401-42’. In total, we identified 18 479 InDel polymorphsms between‘R-O-18’and‘L58’. A total of 500 InDel polymorphisms(4–5 bp in length)were converted to InDel markers and experimentally validated. Of the selected 500 InDel polymorphisms ,452 yielded a single PCR fragment , but only 106 showed polymorphism between‘R-O-18’and‘L58’,48 did not amplify a product,346 showed no polymorphism. These 106 InDel markers were used to screen the genotype of RILs developed from the crossing of ‘R-O-18’and‘L58’. A genetic linkage map contained 99 InDel markers was constructed.

Key words: Brassica campestris, insertion/deletions(InDels), whole genome re-sequencing, Illumina sequencing, genetic linkage map

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