关键词: 桃, PpPGIP1 启动子, 调控元件, 表达特性, 表达载体构建

Abstract: Promoter sequence of PpPGIP1 gene in peach[Prunus persica（L.）Batch]was isolatedthrough the chromosome walking method，and its function was preliminary forecasted by means of bioinformatics method. The plant expression vector of the promoter was constructed and transformed into tobacco through the Agrobacterium-mediated technique. Expression characteristics of PpPGIP1 gene induced by salicylic acid（SA），methyl jasmonate（MeJA），abscisic acid（ABA）and 1-aminocyclopropane-1-carboxylate（ACC）were also analyzed using real-time RT-PCR. The promoter sequence of PpPGIP1
gene with 1 018 bp length was obtained and its homology with that of Prunus mume and Prunus salicina were 90% and 89%，respectively. The promoter contains important cis-acting elements related to resisting pathogens and adversity stress and induced by SA，MeJA，ABA and ETH. The expression of GUS gene could be regulated by PpPGIP1 promoter with the induction of ABA and ACC. The results of real-time RT-PCR indicate that the expression of PpPGIP1 gene could be induced by SA，MeJA，ABA and ACC in the leaves of peach. Thus，PpPGIP1 gene may be involved in the signal transduction pathway of SA，
MeJA，ABA and ETH，and play an important role in the condition of resisting pathogens and adversity stress in peach.

Key words: peach, PpPGIP1 promoter, regulatory element, expression characteristic, construction of expression vector