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园艺学报 ›› 2009, Vol. 36 ›› Issue (12): 1827-1836.

• 综述 • 上一篇    下一篇

DNA序列分析在植物分子系统学研究中的应用现状———以蔷薇科为例

郑小艳;曹家树;滕元文*
  

  1. (浙江大学园艺系, 农业部园艺植物生长发育与品质调控重点开放实验室, 杭州310029)
  • 收稿日期:2009-06-01 修回日期:2009-11-19 出版日期:2009-12-25 发布日期:2009-12-25
  • 通讯作者: 滕元文

Research Progress of Plant Molecular Phylogenetic Analyses Based on DNA Sequence Data—A Case Review in Rosaceae

ZHENG Xiao-yan;CAO J ia-Shu;TENG Yuan-wen*   

  1. (Department of Horticulture, the State Agricultural Ministry Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Hangzhou 310029, China)
  • Received:2009-06-01 Revised:2009-11-19 Online:2009-12-25 Published:2009-12-25
  • Contact: TENG Yuan-wen

摘要: 近20年来, DNA序列已被广泛应用于植物各分类阶元的系统学研究中, 为解决长期有争议
的和亟待解决的系统进化问题提供了有力的证据。现以蔷薇科为例, 概述了应用DNA片段进行植物分子系统研究的现状, 详细剖析了应用DNA序列进行植物系统发育研究时常见的问题及其原因, 提出了对存在多倍化、杂交起源和快速分化等复杂进化史的植物类群进行系统学分析时选用DNA序列的策略和注意事项。

关键词: 蔷薇科, DNA序列, 系统发育, ITS假基因, 低拷贝核基因

Abstract: During the last twenty years, DNA sequences have been widely used in phylogenetic analyses of plants at the different taxonomic levels, and provided convincing evidence to solve the long-standing disputed or unsolved phylogenetic problems. In this paper, the current status of applications of DNA fragments in plant phylogenetic studies were briefly reviewed taking Rosaceae as an examp le, and the common troubles occurring in phylogenetic studies and the possible reasons were discussed in detail. Finally, the strategies and
points for attention in selecting DNA sequences were proposed for phylogenetic reconstruction of plant taxa especially for plants that have undergone complex evolutionary processes such as hybridization, polyploidization and rapid radiation.

Key words: Rosaceae, DNA sequence, phylogeny, ITS pseudogene, Low2copy nuclear gene

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