黄瓜瓜把长度QTL定位的研究

园艺学报 ›› 2008, Vol. 35 ›› Issue (4): 543-546.

黄瓜瓜把长度QTL定位的研究

王桂玲;秦智伟﹡;周秀艳;赵咫云

（东北农业大学园艺学院，哈尔滨 150030 ）

收稿日期:2007-12-10 修回日期:2008-03-07 出版日期:2008-04-25 发布日期:2008-04-25
通讯作者: 秦智伟

Mapping Quantitative Trait Loci Influencing Cucumber Carpopodium Length Using Simple Sequence Repeat Markers

WANG Gui-ling, QIN Zhi-wei﹡, ZHOU Xiu-yan, and ZHAO Zhi-yun

(Department of Horticulture, Northeast Agricultural University, Harbin, 150030, China)

Received:2007-12-10 Revised:2008-03-07 Online:2008-04-25 Published:2008-04-25
Contact: QIN Zhi-wei

摘要/Abstract

摘要： 本研究的主要目的是利用长瓜把的129 与短瓜把的Z3杂交衍生的F2株系，检测与黄瓜瓜把相关的QTL。DNA从F2株系提取，并且利用SSR引物对其进行扩增。总共有5个呈现清晰、可重复的多态性分子标记用于QTL检测。结合瓜把数据，利用Mapmaker 3.0b构建基因连锁图谱，运用Windows QTL Cartographer V2.0检测QTL。LOD大于2作为QTL存在的阈值。4个来自于同一个连锁群的分子标记CSWGATT01B、CSWGATT01C、CSCT335 和CSWGATT01A与瓜把长度显著相关。Qchl1与分子标记CSWGATT01B, CSWGATT01C 紧密连锁，与二者分别相距3.4 cM 和 18.0 cM，且变异贡献率为18.49%。

关键词: 黄瓜, 瓜把长度, SSR, QTL

Abstract:

An F2 population from a cross between 129, a cucumber line with long carpopodium, and Z3, a cultivar with short carpopodium, was used to identify quantitative trait loci (QTLs) associated with cucumber carpopodium length. DNA was extracted from F2 plants and amplified using simple sequence repeat (SSR) marker. A total of five polymorphic SSR markers were used for QTL detection. Based on the data recorded, those five markers were used to construct the genetic linkage map using mapmaker 3.0b. QTLs were detected with Windows QTL Cartographer V2.0. Four markers CSWGATT01B, CSWGATT01C, CSCT335 and CSWGATT01A, from one linkage group, were significantly associated with carpopodium length. Qchl1 was linked with SSR maker CSWGATT01B and CSWGATT01C by 3.4 cM and 18.0 cM, respectively. It explained 18.49% of phenotypic variance.

Key words: Cucumis sativus, carpopodium length, SSR, QTL

中图分类号:

S 642.2

王桂玲;秦智伟﹡;周秀艳;赵咫云 . 黄瓜瓜把长度QTL定位的研究[J]. 园艺学报, 2008, 35(4): 543-546.

WANG Gui-ling;QIN Zhi-wei﹡;ZHOU Xiu-yan;and ZHAO Zhi-yun. Mapping Quantitative Trait Loci Influencing Cucumber Carpopodium Length Using Simple Sequence Repeat Markers [J]. ACTA HORTICULTURAE SINICA, 2008, 35(4): 543-546.

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