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园艺学报 ›› 2006, Vol. 33 ›› Issue (1): 175-178.

• 研究简报 • 上一篇    下一篇

适于制作人工种子的一品红体细胞胚的培养

肖 颖1, 2;王 刚1
  

  1. (1 河北师范大学生命科学学院, 石家庄050016; 2 石家庄学院化学系, 石家庄050801)
  • 收稿日期:2005-04-08 修回日期:2005-06-27 出版日期:2006-02-25 发布日期:2006-02-25

Studies on High Quality Somatic Embryogenesis Applied to Artificial Seed of Euphorbia pulcherrima Willd.

Xiao Ying1, 2; and Wang Gang1   

  1. (1 The College of Life Science, Hebei Normal University, Shijiazhuang 050016, China; 2Department of Chemistry, Shijiazhuang College, Shijiazhuang 050801, China )
  • Received:2005-04-08 Revised:2005-06-27 Online:2006-02-25 Published:2006-02-25

摘要: MS + 2,4-D 2.0 mg·L - 1 + 6-BA 0.5 mg·L - 1固体培养基较适合一品红苞片诱导愈伤组织。MS+ 2,4-D 1.0 mg·L - 1 + 6-BA 0.5 mg·L - 1 +水解酪蛋白80 mg·L - 1固体培养基上体细胞胚分化率最高。活性炭、MS培养基和悬浮振荡培养有利于高频率体细胞胚发生。球形、心形及梨形胚及时转入添加NAA 0.2 mg·L - 1、6-BA 0.5 mg·L - 1及水解酪蛋白80 mg·L - 1的MS液体培养基中, 有利于高质量体细胞胚发生。子叶期胚及时转入添加ABA 0.5 mg·L - 1的上述培养基中, 会提高正常体细胞胚的百分率。用高质量(子叶期) 的一品红体细胞胚包埋制作人工种子, 在无菌条件下萌发率为58.1%。

关键词: 一品红, 愈伤组织, 体细胞胚发生, 人工种子

Abstract: MS agarmedium supp lemented with 2,4-D 2.0 mg·L-1 and 6-BA 0.5 mg·L-1 was proved to be the best for inducing callus from the leaves of Euphorbia pulcherrima Willd. It was suitable for somatic embryogenesis when the concentration of 2,4-D was reduced to 1.0 mg·L-1. Activated carbon, MSmedium and suspension culture were suitable for high frequency somatic embryogenesis. If globular embryoids, heart-shaped embryoids and pear 6-BA shaped embryoidswere transferred intoMSmedium supplemented with NAA
0.2 mg·L -1, 6-BA 0.5 mg·L -1 and casein hydrolyzed 80 mg·L -1 , the quality of somatic embryos would be improved. If cotyleden embryoids were transferred in time into a forementioned medium added on ABA 0.5 mg·L -1 , the rate of normal somatic embryogenesis would be increased.

Key words: Euphorbia pulcherrima, Callus, Somatic embryogenesis, Artificial seed