转化CaMV基因芸薹属蔬菜植株抗病性鉴定及其遗传分析

园艺学报 ›› 2001, Vol. 28 ›› Issue (5): 466-468.

转化CaMV基因芸薹属蔬菜植株抗病性鉴定及其遗传分析

巩振辉¹;何玉科²;宋献军¹;张广辉¹;张桂华¹

( ¹ 西北农林科技大学园艺学院, 杨凌712100; 􀀁² 中国科学院上海植物生理研究所, 上海200032)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2001-10-25 发布日期:2001-10-25

Diagnosis and Genetic Analysis of Resistance to Cauliflower Mosaic Virus inBrassica Crops Which Was Transformed with CaMV Gene VI

Gong Zhenhui¹, He Yuke², Song Xianjun¹, Zhang Guanghui¹, and Zhang Guihua¹

(¹Horticulture College, Northwest Sci􀀁Tech University of Agriculture and Forestry , Yangling 712100; ²Shanghai Institute of Plant Physiology , The Chinese Academy of Science, Shanghai 200032)

Received:1900-01-01 Revised:1900-01-01 Online:2001-10-25 Published:2001-10-25

摘要/Abstract

摘要： 以转化CaMV 弱株系Bari1 基因􀀂 的大白菜、菜薹、紫菜薹和花椰菜植株为试材, 研究了CaMV 弱株系Bari􀀁1 基因􀀂 所提供的遗传工程交叉保护及其遗传规律。结果表明,在转化CaMV 弱株系Bari􀀁1 基因􀀂 的大白菜、菜薹、紫菜薹和花椰菜植株中, 48. 08%对CaMV
强株系CABB􀀁BJI 具有较强的抗性, 51. 92%表现为敏感。抗性基因( CaMV Bari􀀁1 基因) 在自交代( S1) 中大多数( 76%) 表现为典型的孟德尔单基因显性遗传, 部分株系的抗性出现了15 1, 1 1, 1 3 和1 95 的非孟德尔遗传现象。

关键词: CaMV 基因, 芸薹属, 转基因, 植株, 遗传工程交叉保护, 遗传规律

Abstract: The genetically engineered cross􀀁protection which was supplied by weak strain Bari􀀁1 gene VI of cauliflower mosaic virus ( CaMV) , and its genetic regularity were studied with Brassica crops ( Brassica camp estris ssp.
rap ifera, B . campestris ssp. pekinensis , B . campestris var . parachinensis , B. campestris var. purpurea, B .juncea , B . oleracea var. botrytic ) which were transformed with CaMV gene VI. The results showed that 48. 08% of
transformed Brassica plants was resistance to CaMV strain CABB􀀁BJI and 51. 92% of them was susceptible to CaMV CABB􀀁BJI. The virus resistant gene ( CaMV Bari􀀁1 gene VI ) in 76% of first selfed progeny presented typically
Mendelian single dominance gene inheritance. The ratio of virus􀀁resistant to virus􀀁susceptible plants in some of first selfed progeny presented Mendelian inheritance, such as, 15 1, 1 1, 1 3 and 1 95.

Key words: CaMV gene VI, Brassica L., Transgenic, Plants, Genetically engineered cross􀀁, protection, Genetic regularity

中图分类号:

S 634􀀁

巩振辉;何玉科;宋献军;张广辉;张桂华. 转化CaMV基因芸薹属蔬菜植株抗病性鉴定及其遗传分析[J]. 园艺学报, 2001, 28(5): 466-468.

Gong Zhenhui;He Yuke;Song Xianjun;Zhang Guanghui;and Zhang Guihua. Diagnosis and Genetic Analysis of Resistance to Cauliflower Mosaic Virus inBrassica Crops Which Was Transformed with CaMV Gene VI[J]. ACTA HORTICULTURAE SINICA, 2001, 28(5): 466-468.

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