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园艺学报 ›› 2021, Vol. 48 ›› Issue (7): 1304-1316.doi: 10.16420/j.issn.0513-353x.2021-0151

• 研究论文 • 上一篇    下一篇

白菜与埃塞俄比亚芥远缘杂交种质创制及黑腐病抗性转育

张双双, 苏维, 刘阳, 王海平, 宋江萍, 阳文龙, 贾会霞, 张晓辉*(), 李锡香   

  1. 中国农业科学院蔬菜花卉研究所/农业农村部园艺作物生物学与种质创制综合重点实验室,农业农村部蔬菜作物基因资源与种质创制北京科学观测实验站,北京100081
  • 收稿日期:2021-03-23 修回日期:2021-05-21 出版日期:2021-07-25 发布日期:2021-08-10
  • 通讯作者: 张晓辉 E-mail:zhangxiaohui01@caas.cn
  • 基金资助:
    国家重点研发计划项目(2016YFD0100204-07);国家自然科学基金项目(31772301);中央级公益性科研院所基本科研业务费专项(IVF-BRF2020001)

Germplasm Innovation and Black Rot Resistance Transferring in Brassica rapa ssp. chinensis Through Interspecific Hybridization with Brassica carinata

ZHANG Shuangshuang, SU Wei, LIU Yang, WANG Haiping, SONG Jiangping, YANG Wenlong, JIA Huixia, Zhang Xiaohui*(), LI Xixiang   

  1. Key Laboratory of Biology and Genetic Improvement of Horticultural Crops,Ministry of Agriculture and Rural Affairs & Beijing Research Station of Vegetable Crop Gene Resource and Germplasm Enhancement,Ministry of Agriculture and Rural Affairs,Beijing 100081,China
  • Received:2021-03-23 Revised:2021-05-21 Online:2021-07-25 Published:2021-08-10
  • Contact: Zhang Xiaohui E-mail:zhangxiaohui01@caas.cn

摘要:

以白菜(Brassica rapa ssp. chinensis,AA基因组)为母本,以起源于非洲对黑腐病免疫的埃塞俄比亚芥(Brassica carinata,BBCC基因组)为父本进行远缘杂交,通过胚挽救方法获得ABC基因组杂种植株。杂种植株营养体粗壮高大,杂种优势明显。分子标记和流式细胞检测确定是真杂种。F1代植株初期无花粉,组培继代多代后育性逐渐恢复。与埃塞俄比亚芥回交的后代株系育性恢复速度高于与白菜回交的后代。流式细胞分析发现杂种染色质含量介于白菜和埃塞俄比亚芥之间,并且存在染色体和非整倍体加倍现象。花粉母细胞染色体压片显示减数分裂中存在染色体不减数、染色体滞后、染色体非对称分离、染色体粘贴和染色体桥等现象。从人工合成ABC植株与白菜回交的群体中筛选出高抗或免疫黑腐病的株系,抗性可以遗传。

关键词: 白菜, 埃塞俄比亚芥, 远缘杂交, 黑腐病, 种质创新

Abstract:

Present study synthesized the ABC genomic plant by interspecific hybridization and embryo rescue using pakchoi(Brassica rapa ssp. chinensis,AA genome)as maternal and Ethiopian mustard(Brassica carinata,BBCC genome),which originated from Africa and was immune to black rot. The hybrid plant showed strong heterosis at the vegetative growth stage. The genomic composition was confirmed by molecular markers and flow cytometry analysis. The F1 plants were sterile at beginning but gradually restored the fertility after several generation of tissue culture and propagation. The fertility restoration is faster in the backcross progeny with Ethiopian mustard than that with pakchoi. Flow cytometry analysis indicated that the chromosomal contents of the F1 and backcross progeny distributed in the interval of pakchoi and Ethiopian mustard. The automatic chromosomal doubling was observed in the leaf tissue cells. The abnormal chromosome behaviors include non reduction,chromosome lagging,asymmetric segregation,chromosome stickiness and chromosome bridges were revealed in the pollen mother cells. The black rot immune or high resistant lines were screened from the backcross progeny of the synthesized ABC with pakchoi plants. The resistance was inheritable.

Key words: Brassica rapa ssp. chinensis, Brassica carinata, inter-specific hybridization, black rot, germplasm innovation

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