https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2021, Vol. 48 ›› Issue (5): 934-946.doi: 10.16420/j.issn.0513-353x.2020-0772

• 研究论文 • 上一篇    下一篇

SSR标记遗传距离与结球甘蓝杂种优势的关系分析

董艺, 冯羽飞, 许忠民*(), 王世民, 唐鸿吕, 黄炜   

  1. 西北农林科技大学园艺学院,陕西杨凌 712100
  • 收稿日期:2020-11-12 修回日期:2021-03-24 出版日期:2021-05-25 发布日期:2021-06-07
  • 通讯作者: 许忠民 E-mail:xuzhongmin2003@126.com
  • 基金资助:
    国家重点研发计划项目(2016YFD0101702);国家现代农业产业技术体系建设专项资金项目(CARS-23-G22);西安市科技计划项目(20NYTX0001)

Analysis of the Relationship Between Genetic Distance and Heterosis by SSR Markers in Cabbage(Brassica oleracea var. capitata)

DONG Yi, FENG Yufei, XU Zhongmin*(), WANG Shimin, TANG Honglü, HUANG Wei   

  1. College of Horticulture,Northwest A & F University,Yangling,Shaanxi 712100,China
  • Received:2020-11-12 Revised:2021-03-24 Online:2021-05-25 Published:2021-06-07
  • Contact: XU Zhongmin E-mail:xuzhongmin2003@126.com

摘要:

利用SSR标记对23份春甘蓝自交系和29份秋甘蓝自交系进行遗传多样性分析,结果表明:春甘蓝自交系在遗传相似系数0.61处可以划分为极早熟和早熟2个类群。秋甘蓝自交系在遗传相似系数0.58处可以分为中晚熟群、中早熟群和中熟群3个类群。从上述材料中挑选15份代表性材料作为亲本,采用完全双列杂交法组配105个杂交组合,对其进行杂种优势分析,同时探究SSR分子标记遗传距离与杂种优势之间的关系。结果发现,不同类群间杂交或同一类群不同亚群间杂交产生的后代杂种优势较强,遗传距离与全株质量中亲优势的相关性达显著正相关,表明基于SSR分子标记划分的类群对于杂种优势的预测具有一定的价值。此外还发现,在105个杂交组合中,全株质量中亲优势最强的10个组合的组配方式均为春甘蓝 × 秋甘蓝,表明春甘蓝 × 秋甘蓝可能是一种潜在的杂种优势利用模式。

关键词: 甘蓝, 结球甘蓝, SSR标记, 杂种优势, 遗传距离, 聚类分析, 预测

Abstract:

SSR markers were used to analyze the genetic diversity of 23 spring cabbage and 29 autumn cabbage inbred lines. The results showed that the 23 spring cabbage inbred lines were classified into two major groups,namely the extremely early maturing group and the early maturing group at the genetic similarity coefficient of 0.61. Meanwhile,twenty-nine inbred lines of autumn cabbage were classified into three groups,namely middle-late maturing,middle-early maturing,and middle maturing group. Fifteen inbred lines were selected to make 105 hybrid combinations with complete diallel method to analyze their heterosis and explore the relationship between genetic distance and heterosis by SSR markers. At the genetic similarity coefficient of 0.58,the heterosis analysis of 105 hybrid combinations obtained from 15 parental materials meant that the offspring produced by crosses among different groups or between different subgroups of the same group had stronger heterosis. Coefficient among genetic distance based on polymorphism of SSR markers and gross weight mid-parental heterosis was significant at 0.01 level. Thus,the classification based on SSR markers had a certain value for heterosis prediction. In addition,this study also found that among 105 hybrid combinations,the 10 combinations with the strongest mid-parental heterosis among gross weight was spring cabbage × autumn cabbage,indicating that the combination of spring cabbage × autumn cabbage had a potential utilization on heterosis breeding.

Key words: cabbage, SSR marker, heterosis, genetic distance, cluster analysis, prediction

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