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园艺学报 ›› 2021, Vol. 48 ›› Issue (1): 26-36.doi: 10.16420/j.issn.0513-353x.2020-0132

• 研究论文 • 上一篇    下一篇

过氧化氢酶基因CsKat01与柑橘溃疡病相关性分析

祁静静, 秦秀娟, 谢宇, 陈善春, 何永睿*(), 李强*()   

  1. 西南大学/中国农业科学院柑桔研究所,重庆 400712
  • 收稿日期:2020-06-05 修回日期:2020-09-06 出版日期:2021-01-25 发布日期:2021-01-29
  • 通讯作者: 何永睿,李强 E-mail:heyongrui@cric.cn;liqiang@cric.cn
  • 基金资助:
    国家重点研发计划项目(2018YFD1000306);中央高校基本科研业务费项目(SWU115025);广西科技重大专项(桂科AA18118046-6);国家现代农业产业技术体系建设专项资金项目(CARS-26)

Correlation Analysis of Citrus Catalase Gene CsKat01 and Citrus Canker Disease

QI Jingjing, QIN Xiujuan, XIE Yu, CHEN Shanchun, HE Yongrui*(), LI Qiang*()   

  1. Citrus Research Institute,Southwest University/Chinese Academy of Agricultural Sciences,Chongqing 400712,China
  • Received:2020-06-05 Revised:2020-09-06 Online:2021-01-25 Published:2021-01-29
  • Contact: HE Yongrui,LI Qiang E-mail:heyongrui@cric.cn;liqiang@cric.cn

摘要:

为了探究过氧化氢酶基因CsKat01在柑橘抵御溃疡病过程中的作用,对感病品种‘晚锦橙’和抗病品种‘四季橘’中CsKat01进行了生物信息和表达分析,并探究了溃疡病菌接种处理后两品种中CAT酶活性和H2O2含量的关系。克隆CsKat01的编码序列并对该基因及其编码蛋白进行结构和功能分析,发现CsKat01编码框全长为1 482 bp,共编码493个氨基酸残基,蛋白序列中含有典型的CAT结构域;通过启动子序列克隆和分析发现‘晚锦橙’和‘四季橘’核心启动子区域均含有水杨酸、茉莉酸和脱落酸的响应元件,但数量不同;利用qRT-PCR分析水杨酸、茉莉酸、脱落酸和柑橘溃疡病菌(Xcc)对CsKat01的诱导表达特性,发现CsKat01的高表达可能使柑橘相对更感病;结合Xcc诱导后植物组织中CsKat01酶活性和H2O2含量,综合分析CsKat01、CAT酶活、H2O2含量和柑橘对溃疡病抗性之间的关系,推测CsKat01基因可能通过调控CAT的酶活性,改变H2O2含量进而影响柑橘对溃疡病的抗性。

关键词: 柑橘, 溃疡病, 过氧化氢酶, CsKat01, 过氧化氢

Abstract:

In order to investigate the correlation between CsKat01 and citrus bacterial canker disease (CBCD)response,bioinformatics and expression analysis of CsKat01 as well as the relationship between CAT activity and Hydrogen peroxide(H2O2)contents in CBCD susceptible variety Wanjincheng and resistant variety Calamondin were performed. Firstly,the coding sequence(CDS)of the CsKat01 gene was cloned and the structure and function of the coding protein were analyzed. The full-length CDS of CsKat01 is 1 482 bp and it encodes 493 amino acid residues with a typical catalase domain. Secondly,by cloning and analyzing the promoter sequence,we studied the promoter regulatory elements and found that the core promoters of both Wanjincheng and Calamondin contained salicylic acid(SA),jasmonic acid(JA)and abscisic acid(ABA)response elements whose numbers are different. Thirdly,qRT-PCR was used to analyze the induced expression profiles of plant hormones and Xanthomonas citri subsp. citriXcc)on CsKat01,and then the correlation between the enzyme and CBCD was determined. Finally,the relations between CsKat01,CAT activity,H2O2 content and CBCD resistance were analyzed. We speculate that CsKat01 may regulate CAT enzyme activity,which further regulates H2O2 accumulation,and then regulates CBCD resistance.

Key words: citrus, bacterial canker disease, catalase, CsKat01, hydrogen peroxide

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