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园艺学报 ›› 2019, Vol. 46 ›› Issue (8): 1503-1518.doi: 10.16420/j.issn.0513-353x.2018-1025

• 研究论文 • 上一篇    下一篇

芦笋两性花与雄花中发生可变剪接基因的差异分析

黄 玲*,李琼英*,韦树谷,赖 佳,代顺冬,张骞方,曾华兰,刘 佳,叶鹏盛**   

  1. 四川省农业科学院经济作物育种栽培研究所,蔬菜种质与品种创新四川省重点实验室,成都 610300
  • 出版日期:2019-08-25 发布日期:2019-08-25
  • 基金资助:

    四川省财政创新能力提升工程项目(2016ZYPZ-007,2018QNJJ-003)

Identification and Difference Analysis of the Alternative Splicing Event in the Hermaphroditic Flowers and Male Flowers of Asparagus officinalis

HUANG Ling*,LI Qiongying*,WEI Shugu,LAI Jia,DAI Shundong,ZHANG Qianfang,ZENG Hualan, LIU Jia,and YE Pengsheng**   

  1. Industrial Crops Research Institute,Sichuan Academy of Agricultural Sciences;Vegetable Germplasm Innovation and Variety Improvement Key Laboratory of Sichuan,Chengdu 610300,China
  • Online:2019-08-25 Published:2019-08-25

摘要:

可变剪接在植物生长发育过程中起着非常重要的作用,它能使同一个mRNA前体生成多种不同的异构体和产生蛋白质多样性。利用RNA-seq测序技术分析芦笋两性花蕾与雄花蕾性别分化前期、中期、后期的可变剪接,从中发掘与两性花及雄花形成密切相关的特有可变剪接。研究结果显示,在所有试验材料中基因可变剪接均以3′端可变剪切位点(3S)和外显子跳跃(ES)类型为主。GO分析表明,两性花与雄花在3个时期均发生可变剪接的基因主要富集于代谢过程、生物合成以及物质结合相关GO条目中。通过GO和KEGG分析发现,两性花特有的可变剪接基因主要富集到代谢途径、核糖体蛋白等含氮化合物的生物合成代谢、过氧化物酶体、氧化磷酸化途径等,其中含氮化合物的生物合成代谢过程和过氧化物酶等抗氧化活性可能与两性花雌蕊的发育相关。雄花蕾特有的可变剪接基因主要富集到代谢途径、次生代谢产物的生物合成和脂肪酸生物合成等脂质代谢过程,其中脂肪酸生物合成等脂质代谢过程可能与雄花中雌蕊发育有关。

关键词: 芦笋, 两性花, 雄花, 性别分化, 可变剪接

Abstract:

Alternative splicing plays an important role in plant growth and development,which causes the same mRNA precursor for turning into different isoforms and producing protein diversity. To analyze the relationship of alternative splicing of Asparagus officinalis L. and the sex differentiation,the alternative splicing occurring in hermaphroditic flower buds and male flower buds were identified using RNA-seq technology. The results showed that a large number of alternative splicing events were identified in hermaphroditic flower buds and male flower buds. The dominated alternative splicing types were the 3′ splice site selection and exon skipping. The GO annotation showed that the shared alternative splicing genes by hermaphroditic flower buds and male flower buds mainly enriched in metabolic processes,biosynthetic process,cellular components and material-associated entries. The results of GO and KEGG analysis showed that the specific alternative splicing genes were significantly enriched in metabolic pathway,nitrogen compound metabolic process,peroxisome and oxidative phosphorylation pathway for hermaphroditic flower buds. The nitrogen compound metabolic process and the activities of antioxidant enzymes were probably related to the development of the asparagus pistil. For male flowers,the specific alternative splicing genes were significantly enriched in metabolic pathways,biosynthesis of secondary metabolites and the lipid metabolic process such as fatty acid biosynthesis. There was a possibility that the lipid metabolic process could lead to the suspension of pistil development in male flowers of asparagus. The results of this study not only provided a theoretical basis for regulating the formation of hermaphroditic flowers,but also for studying deeply the molecular mechanism of sex differentiation and sex determination in Asparagus officinalis L.

Key words: Asparagus officinalis, hermaphroditic flower, male flower, sex differentiation, alternative splicing

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