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园艺学报 ›› 2019, Vol. 46 ›› Issue (11): 2239-2248.doi: 10.16420/j.issn.0513-353x.2018-1010

• 研究报告 • 上一篇    下一篇

供氮水平对苹果砧木‘M9T337’幼苗生长和GS、GOGAT、AS基因表达的影响

侯 昕,徐新翔,贾志航,于天武,葛顺峰*,姜远茂*   

  1. 山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,山东泰安 271018
  • 出版日期:2019-11-25 发布日期:2019-11-25
  • 基金资助:
    国家重点研发计划项目(2016YFD0201100);国家自然科学基金项目(31501713);国家现代农业产业技术体系建设专项资金项目(CARS-27)

Effect of Nitrogen Supply Levels on Growth and Expression of Glutamine,Glutamate and Asparagine Synthase Genes of‘M9T337’Apple Rootstock

HOU Xin,XU Xinxiang,JIA Zhihang,YU Tianwu,GE Shunfeng*,and JIANG Yuanmao*   

  1. College of Horticulture Science and Engineering,Shandong Agricultural University,State Key Laboratory of Crop Biology,Tai’ an,Shandong 271018,China
  • Online:2019-11-25 Published:2019-11-25

摘要: 以水培条件下苹果砧木‘M9T337’幼苗为试材,研究了低、适宜和高供氮水平下(0.5、5和25 mmol ? L-1)谷氨酰胺合成酶(GS)、谷氨酸合成酶(GOGAT)、天冬酰胺合成酶(AS)基因表达的变化。结果表明,在低氮处理下,根系中GS基因表达量上升幅度明显高于GOGAT和AS,基本趋势是先上调表达后下调表达,叶片中3个基因表达量基本趋于稳定,低氮处理1 d时GOGAT基因在根系中几乎趋于不表达。随着氮素水平的提高,根系中GS和GOGAT基因的表达呈先上调再下调表达的动态变化,叶片中GS和GOGAT基因的表达量升高,AS基因无显著变化,高氮处理下,GS和GOGAT的基因表达受到抑制,GOGAT基因在根系中表达量极低,但叶片表达量明显高于根系表达量。因此,AS和GS参与苹果根系对低氮胁迫的响应,适宜氮水平能够诱导氮代谢关键酶在氮代谢循环中发挥作用,而高氮水平则抑制氮代谢关键酶的基因表达,对植物氮代谢有负面影响。

关键词: 苹果, 砧木, 氮代谢, 供氮水平, 基因表达

Abstract: The gene expression characteristics of glutamine synthetase(GS),glutamate synthetase (GOGAT),asparagine synthetase(AS)of‘M9T337’apple seedlings at different nitrogen levels(0.5,5 and 25 mmol ? L-1)were analyzed in hydroponic conditions. The results showed that,under low nitrogen stress treatment,the increase of GS gene expression in the underground part was significantly higher than that of GOGAT and AS,and the basic trend was up-regulated first and down-regulated later,and the expression levels of three genes in the overground part basically tended to be stable,and the expression of GOGAT in the root system basically tended to be not expressed after 1 d of low nitrogen treatment. With the increase of nitrogen level,the expression of GS and GOGAT genes in roots was up-regulated and then down-regulated,the expression of GS and GOGAT genes in leaves increased,and there was no significant difference in the change of AS. Under high nitrogen treatment,the gene expression of GS and GOGAT was inhibited,and the expression of GOGAT is extremely low in the root system,but the expression level in the overground part was significantly higher than that in the underground part. So,the AS and GS are involved in the response of apple root system to low nitrogen stress,and appropriate nitrogen level can induce the nitrogen metabolism key enzymes play a role in nitrogen metabolism circulation;however,high nitrogen level can inhibit the gene expression of nitrogen metabolism key enzymes in nitrogen metabolism,which had a negative effect on nitrogen metabolism in plants.

Key words: apple, rootstock, nitrogen metabolism, nitrogen supply level, gene expression

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