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园艺学报 ›› 2018, Vol. 45 ›› Issue (7): 1272-1282.doi: 10.16420/j.issn.0513-353x.2018-0050

• 研究论文 • 上一篇    下一篇

缺硼和低pH对枳苗根系细胞壁组分及细胞中硼分布的影响

杜晨晴,吴秀文,闫 磊,刘亚林,姜存仓*   

  1. (华中农业大学资源与环境学院/微量元素研究中心,武汉 430070)
  • 出版日期:2018-07-25 发布日期:2018-07-25

Effect of Boron Deficiency and Low pH on Cell Wall Components and Boron Distribution in Cell of Trifoliate Root

DU Chenqing,WU Xiuwen,YAN Lei,LIU Yalin,and JIANG Cuncang*   

  1. (College of Resources and Environment/Microelements Research Center,Huazhong Agricultural University,Wuhan 430070,China)
  • Online:2018-07-25 Published:2018-07-25

摘要: 采用营养液培养的方法,设置pH值为4、5、6,硼浓度为0(缺硼)和10 μmol ? L-1(适宜硼),探究营养液低pH与柑橘砧木枳[Poncirus trifoliate(L)Raf.]幼苗根细胞壁组分及各组分中硼含量的关系。结果表明:①缺硼和低pH值均抑制根系生长;硼适宜时pH 4处理的根系长度比pH 5和pH 6处理减少了26%和29%,但根系干物质量变化不显著;缺硼时pH 4处理的根系干物质量也显著减少。②硼适宜时pH 4处理的地下部硼含量显著增加,且原生质体硼和游离态硼占根硼的比例均降低;缺硼时pH 4处理的原生质体硼占根硼的比例增加,游离态硼占根硼的比例没有显著变化。③缺硼时,pH 4处理细胞壁提取率显著低于pH 6处理。④不论适宜硼或缺硼,pH 4处理均显著降低根系细胞壁中螯合态果胶含量;适宜硼时碱溶性果胶含量pH 4和pH 5处理分别比pH 6减少了26%和18%;缺硼时pH 4处理比pH 5和pH 6处理减少了约20%。⑤硼适宜时根系纤维素含量随pH值降低而降低,pH 4处理比pH 6处理降低了13.8%;缺硼时低pH对根系纤维素含量影响不大。⑥大部分硼都结合在果胶中,且碱溶性果胶中的硼(Alk-B)的比例大于螯合态果胶(Chel-B)中的。以上结果说明,pH值会通过影响根系细胞壁组分及组分中硼含量而影响根系的伸长生长。

关键词: 枳, pH, 细胞壁, 果胶, 纤维素, 硼含量

Abstract: In order to explore changes of cell wall components and boron distribution under low pH in trifoliate root,the different pHs(4,5,6)and boron concentrations(0 and 10 μmol ? L-1)were set up in experiment. The results showed that boron deficiency and low pH could significantly inhibit root growth and elongation. When the boron concentration was suitable,the root length of pH 4 decreased by 26% and 29% compared with that of pH 5 and pH 6. When boron was absent,root dry weight of pH 4 was significantly reduced. However,when adding boron,the dry matter increase of pH 4 was not significant. When boron was enough,the boron content in the underground part of pH 4 increased significantly;compared with pH 6,the proportion of boron in protoplast and free boron to root boron in pH 4 reduced. Compared with pH 6,the proportion of boron in protoplasts to root boron treated by pH 4 increased and the proportion of free boron to root boron was not significantly changed under boron deficiency. Compared with pH 6,the cell wall extraction rate of pH 4 decreased when boron deficiency. Regardless of boron adequate or boron deficiency,pH 4 significantly decreased the content of chelate pectin in the cell wall of the roots of trifoliate. In the suitable boron treatment,the content of alkali soluble pectin treated by pH 4 and pH 5 decreased by 26% and 18% respectively than that of pH 6. The content of alkali soluble pectin treated by pH 4 decreased by about 20% than that of pH 6 and pH 5 treatments under boron deficiency. In addition,when boron deficiency,low pH had little effect on cellulose content in the root of trifoliate. However,the content of cellulose decreased with the decrease of pH value in suitable boron. Compared with pH 6,the cellulose content of pH 4 decreased by 13.8%. Most of the boron was bound to pectin,and the proportion of boron(Alk-B)in alkali soluble pectin was greater than that of chelated pectin(Chel-B). Obviously,low pH can inhibit the root elongation by affecting the content of boron and components of the root cell wall.

Key words: trifoliate, pH, cell wall, pectin, cellulose, boron content

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