关键词: 紫斑牡丹, 溶血磷脂酸酰基转移酶, 脂肪酸, 三酰甘油

Abstract:

PrLPAAT1 gene was cloned from Paeonia rockii. The expression vector 2S2::PrLPAAT1 was constructed and transformed into wild-type（WT）Arabidposis plants by the floral dip method. Three positive T3 transformants L1OX-11，L1OX-17 and L1OX-19 were confirmed via hygromycin-resistant selection and PCR method. Semi-quantitative RT-PCR analysis indicated that PrLPAAT1 was highly expressed in transgenic Arabidopsis lines. Phenotypic analysis suggested that the seed yield had no significant changes in transgenic Arabidopsis lines，compared to WT plants. We analyzed the content and composition of FAs in mature seeds of the transgenic Arabidopsis using GC–MS. Compared with WT plants，seeds of L1OX-11，L1OX-17，and L1OX-19 line showed increased total fatty acids（FAs）content of 5.3%，and 5.2%，and the oleic acid（OA）content of 19.9%，24.6%，and 19.4%，respectively. Quantitative real-time PCR analysis indicated that a few endogenous genes（AtACP1，AtDGAT1，and AtSUS3）involved in lipid biosynthesis were up-regulated in PrLPAAT1-overexpressing lines. These results showed that PrLPAAT1 may play an important role in fatty acid biosynthesis of seed.