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园艺学报 ›› 2018, Vol. 45 ›› Issue (3): 482-492.doi: 10.16420/j.issn.0513-353x.2017-0369

• 研究论文 • 上一篇    下一篇

西瓜miR164b靶基因鉴定及其对黄瓜绿斑驳花叶病毒侵染应答的分析

孙玉燕,何艳军,牛晓伟,崔 狄,范 敏*   

  1. (浙江省农业科学院蔬菜研究所,杭州 310021)
  • 出版日期:2018-03-25 发布日期:2018-03-25

Identification of the Targets of miR164b and Their Response to Cucumber green mottle mosaic virus Infection in Watermelon

SUN Yuyan,HE Yanjun,NIU Xiaowei,CUI Di,and FAN Min*   

  1. (Institute of Vegetables,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)
  • Online:2018-03-25 Published:2018-03-25

摘要: 将miR164b序列与西瓜基因组进行比对,获得miR164b前体Pre-miR164b基因并克隆。Pre-miR164b长度为97 bp,含有完整的茎环结构。Pre-miR164b启动子区含有光响应元件、赤霉素响应元件、乙烯响应元件、水杨酸响应元件、真菌诱导响应元件等多个顺式作用元件。降解组测序获得miR164b的4个靶基因,均注释为NAC转录因子基因,剪切位点位于miR164b序列5′ 端第10位碱基。miR164b及靶基因在黄瓜绿斑驳花叶病毒(CGMMV)侵染不同时间的表达模式不同,靶基因Cla018596的表达模式与miR164b呈负相关,而Cla019099、Cla023219和Cla023357的表达模式与miR164b没有相关性。

关键词: 西瓜, miR164, NAC, 降解组测序, 表达模式

Abstract: The precursor gene of miR164b,Pre-miR164b,was obtained through blast of miR164b and the watermelon genome. Pre-miR164b,with a length of 97 bp,was able to form the stem-loop structure. The promoter of Pre-miR164b contains several cis-acting regulatory elements,such as light responsive element,gibberellin responsive element,ethylene responsive element,salicylic acid responsive element,and fungal elicitor responsive element. Four target genes of miR164b,annotated as NAC transcription factor,were predicted and the cleavage site was located at the 10th of miR164b at the 5′ end. Expression patterns of miR164b and NAC were different during the process of Cucumber green mottle mosaic virus(CGMMV)infection. Expression of Cla018596 was negatively correlated with miR164b. However,the expression of Cla019099,Cla023219 and Cla023357 was not correlated with miR164b.

Key words: watermelon, miR164, NAC, degradome sequencing, expression pattern

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