关键词: 莲雾, 转录组, SSR, 多态性

Abstract: 87 538 Unigenes were obtained by transcriptome sequencing analysis from wax apple [Syzygium samarangense（Bl.）Merr. et Perry]. 21 153 unigenes above 1 kilo base pairs were analyzed by MISA software，and a total of 11 641 SSRs were identified from 8 115 Unigenes. The frequency of SSRs from these Unigenes was 55.03%，and the mean distribution distance of loci was 4.08 kb. Meanwhile，the major types such as mononucleotide，dinucleotide and trinucleotide accounted for 43.44%，30.98% and 24.38%，respectively. Furthermore，AG/CT was the predominant dinucleotide repeat type（26.99%），and the CCG/CGG was the predominant trinucleotide repeat type. 25 734 pairs of SSR primers were found by Primer 3.0. Thirty-five efficient pairs of primers were randomly selected from 94 valid pairs for PCR amplification by 30 wax apple samples，and 25 PCR products showed clear and reproducible results indicating the polymorphism among these samples. According to the UPGMA analysis，30 plants were divided into 2 groups. Abundant SSR polymorphisms provide reliable markers for genetic map construction and analysis of genetic polymorphism for wax apple.

Key words: wax apple, transcriptome, simple sequence repeat, polymorphism