https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2017, Vol. 44 ›› Issue (7): 1299-1308.doi: 10.16420/j.issn.0513-353x.2017-0168

• 研究论文 • 上一篇    下一篇

白菜翻译起始因子eIF(iso)4E.a/c 与芜菁花叶病毒TuMV-C4/UK1 蛋白互作分析

李国亮*,钱 伟*,张淑江,李 菲,章时藩,张 慧,谢露露,武 剑,王晓武,孙日飞**   

  1. 中国农业科学院蔬菜花卉研究所,北京 100081
  • 出版日期:2017-07-25 发布日期:2017-07-25

Analysis of the Protein Interaction of eIF(iso)4E.a/c with TuMV-C4/UK1 in Brassica rapa ssp. chinensis

LI Guoliang*,QIAN Wei*,ZHANG Shujiang,LI Fei,ZHANG Shifan,ZHANG Hui,XIE Lulu,WU Jian,WANG Xiaowu,and SUN Rifei**   

  1. Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China
  • Online:2017-07-25 Published:2017-07-25

摘要:

为阐明白菜翻译起始因子异构体eIF(iso)4E 的表达特性,以及该异构体与芜菁花叶病毒
(TuMV)的互作关系,在白菜‘极早春’中克隆了eIF(iso)4E.a 基因,在‘BP8407’中克隆了eIF(iso)4E.c
基因。这两个基因长度一致,均编码200 个氨基酸。酵母双杂交和双分子荧光互补试验表明:eIF(iso)4E.a
仅与TuMV-C4 株系互作,而不与TuMV-UK1 株系互作;eIF(iso)4E.c 仅与TuMV-UK1 株系互作,而不与
TuMV-C4 株系互作。对白菜eIF(iso)4E 蛋白氨基酸序列及空间结构分析发现:eIF(iso)4E 蛋白包含帽结合
蛋白结构,eIF(iso)4E.a 和eIF(iso)4E.c 蛋白间存在20 个差异氨基酸,其中17 个差异氨基酸位于帽结合蛋
白结构上;TuMV-C4 VPg 与TuMV-UK1 VPg 蛋白有4 个氨基酸差异位点。通过对TuMV 不同株系的VPg
蛋白(病毒基因组连接蛋白)氨基酸序列频率分析发现,在4 个氨基酸差异位点处,氨基酸的使用频率
具有选择性。

关键词: 白菜, eIF(iso)4E, TuMV, VPg(virus genome-linked protein), 互作关系

Abstract:

To clarify the expression characteristics of eukaryotic initiation factors [eIF(iso)4E] and
the interaction relationship of eIF(iso)4E with TuMV VPg,the eIF(iso)4E.a and eIF(iso)4E.c genes were
cloned from the Brassica rapa ssp. chinensis materials‘Jizaochun’and‘BP8407’,respectively. Both of
the two genes could encode 200 amino acids. The yeast two-hybridand bimolecular fluorescence
complementation(BiFC)results showed that TuMV-C4 could only interact with eIF(iso)4E.a,instead of
eIF(iso)4E.c;the TuMV-UK1 could only interact with eIF(iso)4E.c,instead of eIF(iso)4E.a. Analyzing the amino acid sequence and the domain tertiary structure of eIF(iso)4E protein revealed that it contained the
cap-reception site. There were 20 different amino acids between eIF(iso)4E.a and eIF(iso)4E.c,17 of
which were located in the cap-reception site(accounting for 85%). Through aligning the TuMV-C4/UK1
VPg amino acid sequences,four different amino acids were found;and after comparative analyzing the
sequences from different TuMV strains,the frequency of the amino acids had certain selectivity in the four
amino acid points.

Key words: Brassica rapa ssp. chinensis, eIF(iso)4E, TuMV, VPg(virus genome-linked protein), protein interaction