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园艺学报 ›› 2017, Vol. 44 ›› Issue (6): 1038-1048.doi: 10.16420/j.issn.0513-353x.2017-0010

• 研究论文 • 上一篇    下一篇

5–氨基乙酰丙酸缓解‘红颜’草莓盐胁迫伤害的时间效应研究

吴雯雯,安玉艳,汪良驹*   

  1. (南京农业大学园艺学院,南京 210095)
  • 出版日期:2017-06-25 发布日期:2017-06-25

Study on Time Effects of Exogenous 5-Aminolevulinic Acid Treatment on Alleviating Salinity Injury in‘Benihoppe’Strawberry

WU Wenwen,AN Yuyan,and WANG Liangju*   

  1. (College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China)
  • Online:2017-06-25 Published:2017-06-25

摘要:

为了明确外源5–氨基乙酰丙酸(5-ALA)缓解草莓盐胁迫伤害的适宜处理时间,比较了不同时间点添加外源5-ALA缓解植物盐胁迫伤害的效应,发现在100 mmol ? L-1 NaCl处理前5 d、处理同时或处理后3 d分别用10 mg ? L-1 5-ALA溶液浇灌‘红颜’草莓根际,前5 d预处理的效果最佳,喻示着5-ALA的预防效应高于治疗效应。进一步于盐处理前5、10、15和20 d分别用5-ALA溶液根灌草莓苗,发现提前20 d预处理的效果最好,说明至少在20 d以内提前预处理越早,越有利于缓解盐胁迫对草莓苗的伤害。试验观察到,5-ALA缓解草莓盐胁迫伤害主要表现为:(1)缓解盐胁迫导致的草莓叶片叶绿素含量以及叶绿素b/a比值下降;(2)诱导盐胁迫下草莓叶片和根系抗氧化酶活性上升,缓解由盐胁迫导致的超氧阴离子生成速率加快;(3)有利于PSⅡ–PSⅠ之间电子传递,提高光能吸收、捕获、传递效率,减少热耗散,提高光合性能指数;(4)缓解盐胁迫对草莓植株生长的抑制作用,提高植株整体,特别是根系的生物学产量。

关键词: 草莓, 5-ALA, 盐胁迫, 叶绿素荧光, 活性氧代谢, 时间效应

Abstract:

In order to determine the suitable time for 5-aminolevulinic acid(5-ALA)application to alleviate salinity injury in strawberry(Fragaria × ananassa Duch.‘Benihoppe’),exogenous 10 mg ? L-1 5-ALA solution was rhizospherically applied at 5 days before,simultaneously or 3 days after 100 mmol ? L-1 NaCl treatment,and the results showed that the alleviating effect was best in the pretreatment. This result implies that 5-ALA is more preventive than therapeutic for strawberry against salt stress. After then,another experiment was carried out,where 10 mg ? L-1 5-ALA solutions were rhizospherically pretreated to strawberry plants 5,10,15 or 20 days before NaCl treatment and the result showed that the pretreatment prior 20 d was the best effective treatment,which means that the earlier the strawberry was pretreated with 5-ALA,the less of salinity injury was found. It was observed that the possible alleviating mechanisms of 5-ALA against salt stress in strawberry may include the follows. (1) 5-ALA alleviated the decrease of leaf chlorophyll content and Chl.b/a ratio induced by salinity in strawberry. (2) 5-ALA induced the additional increase of antioxidant enzyme activities and decreased the production rate of superoxide anion in the leaves and roots of strawberry under salt stress. (3) 5-ALA was beneficial to intersystem electron transport from PSⅡ to PSⅠ,increasing the energy conversion efficiency during light energy absorption,harvest and transport,reducing heat dissipation,and improving photosynthetic capacity under salt stress. (4) 5-ALA alleviated the inhibition of plant growth induced by salt stress,including shoots and roots,especially the latter.

Key words: strawberry, 5-ALA;salt stress, chlorophyll fluorescence, reactive oxygen metabolism, time effect

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