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园艺学报 ›› 2016, Vol. 43 ›› Issue (4): 663-673.doi: 10.16420/j.issn.0513-353x.2016-0036

• 果树 • 上一篇    下一篇

紫外光对‘北陆’越橘转色果花青苷积累、关键酶活性及其基因表达的影响

杨俊枫,史文君,杨 乐,宫中志,李彬彬,侯智霞*   

  1. (北京林业大学省部共建森林培育与保护教育部重点实验室,蓝莓研究与发展中心,北京100083)
  • 出版日期:2016-04-25 发布日期:2016-04-25

Effects of UV Irradiation on Accumulation of Anthocyanins and the Activity,Expression of Key Enzyme in Veraison‘Northland’Blueberry

YANG Jun-feng,SHI Wen-jun,YANG Le,GONG Zhong-zhi,LI Bin-bin,and HOU Zhi-xia*   

  1. (Key Laboratory for Silviculture and Conservation Attached to China Ministry of Education,Blueberry Research and Development Center,Beijing Forestry University,Beijing 100083,China)
  • Online:2016-04-25 Published:2016-04-25

摘要: 以3年生‘北陆’越橘为试材,对发育过程中果实花青苷含量及合成酶活性变化进行研究,并进一步利用3种紫外光(UV-A、UV-B、UV-C)分别照射转色期越橘果实,测定了果实中花青苷等酚类物质的含量、生物合成酶活性及其基因表达,从底物、酶活性、基因转录水平阐明了不同紫外光对花青苷生物合成途径的影响。3种紫外光都明显诱导了果实花青苷的积累,特别是UV-C照射后果实花青苷含量提高了2.36倍。UV照射显著诱导果实PAL、UFGT酶活性升高,VcPAL、VcUFGT转录增强,但同时也抑制了DFR酶活性及基因表达,其中PAL、UFGT酶活性与花青苷的积累极显著正相关(r = 0.807**,r = 0.894**),而DFR酶则相反(r =–0.854**)。结果表明,UV照射能诱导丙苯氨酸途径中响应紫外处理的一些关键基因的转录激活(如VcPAL,VcUFGT)或抑制(如VcDFR),并影响相应酶活性的变化,促使花青苷等酚类物质的积累。

关键词: 越橘, 转色期果实, 紫外光, 花青苷, 酚类物质, 酶活性, 基因表达

Abstract:

This experiment was based on the changes between the content of anthocyanins and the activities of biosynthetic enzymes in blueberry during the developmentwhich aimed at further testing accumulations of anthocyanins and other phenolic compounds in blueberry and the enzyme activitiestranscriptional level of anthocyanin biosynthetic gene under the environmental stimulus of UV-AUV-BUV-C with the veraison fruit of 3-year old blueberryNorthland. The results indicated that three types of UV irradiation both significantly induced the accumulation of anthocyanins in blueberryespecially UV-Cincreased by 236% after irradiation. In additionthe activities of PALUFGT and the expression of VcPALVcUFGT mRNA were induced by UV irradiationbut the enzyme activitytranscriptional level of VcDFR were suppressed. Among themPALUFGT enzyme activities were significantly positively correlated with the accumulation of anthocyaninr = 0.807**r = 0.894**),while DFR was the oppositer =0.854**. The results suggest that UV irradiation can induced some key geneswhich responded to UV treatmenttranscriptional enhancementsuch as VcPALVcUFGTor inhibitionsuch as VcDFR),and affected the changes of the corresponding enzyme activitieswhich resulted in the accumulation of anthocyanin and other phenolic compounds.

Key words: blueberry, anthocyanin;UV, veraison fruit, phenolic compound, enzyme activity, gene expression

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