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园艺学报 ›› 2016, Vol. 43 ›› Issue (7): 1377-1382.doi: 10.16420/j.issn.0513-353x.2016-0013

• 研究报告 • 上一篇    下一篇

Ca2+和Cu2+对刺梨果实AsA代谢相关基因表达的影响

李良良,安华明*   

  1. (贵州大学农学院,贵州省果树工程技术研究中心,贵阳 550025)
  • 出版日期:2016-07-25 发布日期:2016-07-25

Effects of Exogenous Divalent Cations Ca2+ and Cu2+ on Expression of Genes Involved in Ascorbate Metabolism in Rosa roxburghii Fruits

LI Liang-liang and AN Hua-ming*   

  1. (Agricultural College,Guizhou University,Guizhou Engineering Research Center for Fruit Crops,Guiyang 550025,China)
  • Online:2016-07-25 Published:2016-07-25

摘要:

以‘贵农5号’刺梨(Rosa roxburghii Tratt)为材料,采用离体浸泡处理的方法研究了外源Ca2+和Cu2+两种金属离子对刺梨果实抗坏血酸(AsA)含量及其相关代谢基因表达的影响。结果表明:Ca2+对刺梨果实中AsA的积累有促进作用,而Cu2+则引起AsA含量明显降低。实时荧光定量PCR分析表明,添加Ca2+对L–半乳糖途径中的基因GalLDHGPP以及再生途径中的基因DHARMDHAR的表达有显著的上调作用,说明Ca2+参与了刺梨果实AsA合成和代谢相关基因转录水平的调控;而外源Cu2+除对AsA合成途径的GalLDHGGP两个基因表现出较小幅度的下调作用外,对分解代谢过程的基因并未有明显的上调效应,意味着Cu2+并不是主要在基因转录水平促进AsA的氧化。

关键词: 刺梨, Ca2+, Cu2+, 抗坏血酸, 基因表达

Abstract:

Fruits of Rosa roxburghii Tratt‘Guinong 5were used to investigate the effects of divalent cations of Ca2+ and Cu2+ on expression of the genes involved in L-ascorbic acid(AsA)biosynthesis and accumulation using the methods of incubation in vitro. The results showed that exogenous Ca2+ had a positive role in promoting AsA accumulation,while Cu2+ accelerated AsA degradation during incubation. Quantitative real-time PCR analysis showed that the expression of GalLDH and GPP in L-galactose biosynthetic pathway and DHAR and MDHAR in recycling pathway upregulated sigificantly when fruit flesh tissues was incubated with Ca2+,indicating that this divalent cation took part in the regulation of gene transcription. Exogenous Cu2+ showed a slight exhibition of GalLDH and GGP expression but failed to down-regulate the expression of AAO or APX,which suggests that AsA degradation incubating Cu2+ occuerrd not primarily on the transcription level.

Key words: Rosa roxburghii, Ca2+, Cu2+, ascorbic acid, gene expression

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