关键词: 苹果, K+转运蛋白, 基因家族, 生物信息学, 表达特性

Abstract:

In this study，the information that contained phylogenetic relationship and distribution of apple potassium（K⁺）transporter genes was analyzed based on Malus genome database，and their expression characteristics were explored by means of qRT-PCRs in apple. The results showed that 65 K⁺ transporter genes were identified，and they were divided into 4 families：CHX family，HAK family，HKT family and KEA family，including 33，24，1 and 7 members，respectively. These genes are highly homologous with Arabidopsis thaliana K⁺ transporter genes. Chromosomal localization exhibited that these K⁺ transporter genes were distributed in the thirteen of seventeen apple chromosomes unevenly. Quantitative expression analysis showed that most of the K⁺ transporter genes had differential expression patterns. The thirty-two genes had higher expression abundance in root while 12 genes in mature leaves，11 genes in shoot tips and 19 genes in fruit. The results lay a basis for revealing the function of K⁺ transporter genes in apple.

Key words: Malus × domestica, potassium transporter, gene family, bioinformatic, expression characteristic