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园艺学报 ›› 2016, Vol. 43 ›› Issue (3): 485-495.doi: 10.16420/j.issn.0513-353x.2015-0898

• 蔬菜 • 上一篇    下一篇

中国加工番茄资源遗传多样性分析

刘希艳,郑 峥,邓学斌,冯晶晶,白金瑞,宋 燕,刘 磊,李君明*   

  1. 中国农业科学院蔬菜花卉研究所,北京 100081
  • 出版日期:2016-03-25 发布日期:2016-03-25
  • 基金资助:
    国家自然科学基金项目(31171962);公益行业(农业)科研专项经费项目(201303115);‘十二五’国家科技支撑计划项目(2012BAD02B04);中国农业科学院科技创新工程项目(CAAS-ASTIP-IVFCAAS);农业部园艺作物生物学与种质创新重点实验室项目

Analysis the Genetic Diversity of Processing Tomato in China

LIU Xi-yan,ZHENG Zheng,DENG Xue-bin,FENG Jing-jing,BAI Jin-rui,SONG Yan,LIU Lei,and LI Jun-ming*   

  1. Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China
  • Online:2016-03-25 Published:2016-03-25

摘要: 基于构建的加工番茄核心种质,从3 026份自交系中选择了代表50年育种历程的615份,分别利用16个表型性状和81个(13对SSR、24对InDel、44个SNP)不同类型的多态性分子标记,对其遗传多样性进行了分析。结果表明,无论是基于表型还是基因型数据,供试自交系聚类结果基本一致,均分为两大群。其中表型聚类包括7个亚群,基因型聚类包括6个亚群。综合分析表型和基因型聚类结果,发现大多数早期(2010年以前)育成的自交系聚为一类,近年(2010年以后)育成的自交系不同程度地分布在两类中,说明近年育成的自交系遗传多样性更为丰富。在不同类型标记中,SNP在亚群中呈现更好的多态性,等位基因数、基因多样性、多态性信息量均明显高于SSR和InDel。

关键词: 加工番茄, 分子标记, 多态性, 遗传变异

Abstract: Based on the information of the established core collection,genetic diversity of 615 typical accessions representing 50 years of breeding program selected from a collection of 3 026 processing tomato inbred lines was analyzed and compared by using 16 phenotypes and 81 polymorphic markers(13 SSR,24 InDel and 44 SNP). The results indicate that all inbred lines were divided into two groups by either phenotypes or genotypes,and show the consistent results by each other. Furthermore these two groups could be classified into 7 and 6 sub-groups respectively. When compared to the categories respectively obtained from both phenotypes and genotypes,we found that most of the early released inbred lines were clustered in groupⅠwhile the developed modern lines were involved randomly in both groupⅠandⅡ. It hints that the newly inbred lines showed much more diverse than the old population. In addition,SNP markers show more polymorphic than SSR and InDel markers as indicated by allele number,gene diversity and polymorphism information content.

Key words: processing tomato, molecular marker, polymorphism, genetic variation

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