https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2015, Vol. 42 ›› Issue (11): 2215-2222.doi: 10.16420/j.issn.0513-353x.2015-0539

• 蔬菜 • 上一篇    下一篇

铅胁迫对姜叶片活性氧代谢的影响

刘灿玉,王 允,张 逸,曹逼力,徐 坤   

  1. 山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,农业部黄淮地区园艺作物生物学与种质创制重
    点实验室,山东果蔬优质高效生产协同创新中心,山东泰安 271018
  • 出版日期:2015-11-25 发布日期:2015-11-25
  • 基金资助:

    山东省现代农业产业技术体系专项资金项目(SDAIT-02-022-05)

Effects of Pb Stress on Reactive Oxygen Metabolism in Ginger Leaves

LIU Can-yu,WANG Yun,ZHANG Yi,CAO Bi-li,and XU Kun   

  1. College of Horticulture Science and Engineering,Shangdong Agricultural University,State Key Laboratory of Crop
    Biology,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops in Huanghuai Region,Ministry of
    Agriculture,Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production,Tai’an,
    Shandong 271018,China
  • Online:2015-11-25 Published:2015-11-25

摘要:

以‘莱芜大姜’为试材,采用污灌方式研究不同水平铅(Pb)胁迫(土壤Pb 含量分别为0、
150、450 和1 350 mg · kg-1)对姜叶片活性氧代谢的影响。结果表明,随Pb 胁迫水平的升高及胁迫时间
的延长,姜叶片中生成速率和H2O2 含量显著升高,但过度Pb 胁迫则使其降低,而丙二醛(MDA)含
量和电解质渗漏率则持续增加。Pb 胁迫40 d 时,超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化
氢酶(CAT)、谷胱甘肽还原酶(GR)、抗坏血酸过氧化物酶(APX)、单脱氢抗坏血酸还原酶(MDHAR)、
脱氢抗坏血酸还原酶(DHAR)等抗氧化酶活性随Pb 胁迫水平的升高显著增强,还原型抗坏血酸(AsA)、
总抗坏血酸(AsA + DHA)及还原型谷胱甘肽(GSH)、总谷胱甘肽(GSH + GSSG)等抗氧化物质含量
显著升高,AsA/DHA、GSH/GSSG 比值显著增加;随胁迫时间的增加,抗氧化酶活性及抗氧化物质含量
则多以150 mg · kg-1 的轻度Pb 胁迫的较高,过度的Pb 胁迫则使其显著降低,表明AsA-GSH 循环遭受严
重破坏。

关键词: 姜, Pb 胁迫, 活性氧代谢

Abstract:

The effects of the different Pb content(0,150,450,1 350 mg · kg-1)stress on the active
oxygen metabolism in ginger leaves were studied with a ginger(Zingiber officinale Roscoe)cultivar
‘Laiwu Ginger’by using the method of sewage irrigation. The results showed that with the increase of Pb
stress level and exposure duration, productivity rate and H2O2 content increased significantly and then
decreased under excessive Pb stress,while MDA content and electrolyte leakage rate increased
continuously. After 40 days of Pb stress which was during the seedling stage,with the increase of Pb stress
level,the activities of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT),glutathione
reductase(GR),ascorbate acid(APX),monodehydroascorbate reductase(MDHAR)and dehydroascorbate
reductase(DHAR)were enhanced markedly,and the contents of reduced ascorbic acid(AsA),totalascorbic acid(AsA + DHA),reduced glutathione(GSH)and total glutathione(GSH + GSSG)were
increased remarkably,and the ratios of AsA/DHA,GSH/GSSG were also increased significantly.
Moreover,with ginger growth going on and the stress time increasing significantly,the antioxidant
enzymes activities and the antioxidant substance contents were higher mostly under 150 mg · kg-1 mild Pb
stress and inhibited under excessive Pb stress,which indicated that the AsA-GSH cycle suffered serious
damage.

Key words: ginger, Pb stress, reactive oxygen metabolism

中图分类号: